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Transfection Protocols & Support
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  • General Transfection Protocols
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General Transfection Protocols

  • Transfecting Plasmid DNA into Mammalian Cells using Lipofectamine® LTX


Cell-type Specific Transfection Protocols (* With the exception of CHO-K1, HeLa-S3, HEK 293, and MCF-7 cells, We found that the use of Plus Reagent (Cat# 11514-015) together with Lipofectamine LTX further enhanced transfection in the rest of the cell lines.

Cell Type Source
A549 Human lung carcinoma
ACHN Human renal cancer
C6 Rat glioma
Caco-2 Human colorectal adenocarcinoma
CHO-K1 * Hamster ovary
COS-7L Monkey kidney fibroblast
GripTite™ 293 MSR Human embryonic kidney
HCT-116 Human colon carcinoma
HEK 293 * Human embryonic kidney
HeLa-S3 * Human cervical carcinoma
HepG2 Human liver carcinoma
HL60 Human lymphoblast
HT29 Human colon adenocarcinoma
HT-1080 Human fibrosarcoma
HUVEC Human umbilical vein cord
IMR-90 Human embryonic lung
Jurkat Human T-cell leukemia
K-562 Human myelogenous leukemia
LNCap Human prostate cancer
MCF-7 * Human breast cancer
MSA-MB-231 Human breast adenocarcinoma
MDA-MB-435 Human breast adenocarcinoma
Molt-4 Human T lymphoblast
NCI-H460 Human large cell lung cancer
NHFF Human diploid somatic fibroblast
NIH-3T3 Mouse embryonic fibroblast
NTera2 Human embryonic carcinmoa stem cell
PC-3 Human prostate adenocarcinoma
PC12 Rat neuronal
SK-BR3 Human breast carcinoma
SK-MEL-28 Human malignant melanoma
SK-OV-3 Human ovarian adenocarcinoma
THP-1 Human monocytic leukemia
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