|Cotransfection refers to the simultaneous transfection with two separate nucleic acid molecules, such as plasmid DNA and siRNA. Cotransfection is a common procedure for stable transfection. The plasmid DNA may contain a gene that is easily assayed and acts as a marker. |
For this to occur, a marker gene is co-transfected, which gives the cell a selection advantage, for example resistance towards a certain toxin. Selecting the right reagent for co transfection is essential.
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Cotransfection Protocol with Lipofectamine® 2000
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