Transfection Reagents

Guaranteed confidence that your DNA, siRNA, oligonucleotides, and RNA get into the cell, Invitrogen™  transfection reagents


  • Superior delivery - transfect a broad range of cell types, including those notoriously difficult to transfect, with high  efficiency
  • Nontoxic performance - you see results due to the presence of your nucleic acid, not the reagent
  • Less optimization - rapid, simple protocols provided for most cell lines

Lipofectamine® Transfection Reagents

Since launching in 1993, Lipofectamine® reagents have become the most referenced  transfection reagents with over 50,000 citations to date. Lipofectamine®  reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.

Transfection ReagentKey Features & Applications of Reagent
Lipofectamine® LTX Transfection Reagent       
  • High transfection efficiency and significantly lower toxicity levels for a wide range of cell lines
  • Significantly improved transfection performance in a number of primary and hard-to-transfect cell lines
  • Optimized protocols for over 30 different cell lines are available so much less time is needed for evaluation and optimization
ExpiFectamine® 293 Transfection Kit
  • Designed for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
  • Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
  • Provides robust and reproducible transfection results
  • Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
FreeStyle® MAX Transfection Reagent
  • Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells
  • Used for a large-scale transient protein production with milligrams of protein yield
Lipofectamine® RNAiMAX Transfection Reagent
  • Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects
  • Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
  • Compatibility with a broad range of cell types providing the most versatile approach to all of your gene silencing experiments
Lipofectamine® 2000 Transfection Reagent
  • Achieves highest efficiency and expression results for plasmid or RNAi transfections
  • Works effectively with many cell types (adherent or suspension)
  • Easy and fast protocol – no need for wash steps before or after transfection
  • Complexes can be added to cells growing in serum-containing media
  • Ideal if transfecting at confluencies of 90% or greater (minimizes cytotoxicity following transfection)
Lipofectamine® 2000 CD Transfection Reagent
  •  Same performance as Lipofectamine® 2000, certified animal-origin free (“CD” = chemically defined)
Oligofectamine® Transfection Reagent
  • Transfection of antisense oligonucleotides
293fectin™ Transfection Reagent
  • Used for transient protein production in combination with the FreeStyle™ 293 Expression System
  • Optimized for suspension FreeStyle™ 293-F cells
Cellfectin® Transfection Reagent
  • Optimal transfection of insect cells, including S2, Sf9, Sf21 and High Five™ cells
DMRIE-C Transfection Reagent
  • Transfection of suspension cells, including CHO, lymphoid and Jurkat cell lines.
Lipofectamine® Transfection Reagent
  • First generation reagent for plasmid DNA transfections
  • In most cases, Lipofectamine® 2000 provides better performance than Lipofectamine® Transfection Reagent
Lipofectin® Transfection Reagent
  • First generation reagent for plasmid DNA transfections
  • In most cases, Lipofectamine® 2000 provides better performance than Lipofectin® Transfection Reagent
Optifect™ Transfection Reagent
  • Broad use reagent designed for low confluency applications (<70% confluent at the time of transfection)
  • Useful for cell lines that are sensitive to transfection reagents