Introduction to Cutaneous Biology
|Human skin is the largest organ of the body. Together with various other components such as glands, fingernails, and hair, it comprises a complex system known as the integumentary system. Human skin performs a number of diverse functions critical to normal human health, including protection from environmental insults such as pathogens, physical damage, and radiation from the sun. |
In addition, skin helps maintain homeostasis, possesses metabolic activity, delivers touch, heat, and pain sensations via the peripheral nervous system, excretes salts and wastes, and aids in wound healing. Primary skin cells are used in a variety of research and clinical applications:
Components of Skin
Healthy skin also contains a number of other structural elements, including collagen fibers and fibroblasts located in the basement membrane, lending strength and structure to the skin. Layered within the basement membrane matrix are dermal microvasculature and lymphatic vessels for blood circulation and waste removal, vital to proper skin function.
Applications for Cutaneous Cell SystemsResearch applications for cutaneous cell systems are shown in Table 1.
|Table 1 - Applications for cutaneous cell systems|
|Basic structure / function studies|
|Drug discovery / cosmetics / beauty and personal care studies|
|In vitro alternatives to animal testing|
|A. Human dermal fibroblasts||B. Human epidermal keratinocytes||C. Human dermal microvascular endothelial cells||D. Human epidermal melanocytes|
Key dermal cell culture products
When you demand robust and relevant tools for your primary cell culture work, select from these and other key dermal cell culture products (Table 2) or visit our primary cell culture homepage.
Table 2 - Products for dermatological research*
|Primary human cells (cryopreserved)||Basal Media||Growth Supplements|
Animal product free prepared
|EpiLife® medium (500 ml)|
- Calcium- and phenol red-free
|Human keratinocyte growth supplement (HKGS)|
EpiLife defined growth supplement (EDGS)
|Melanocytes||- Neonatal, lightly pigmented donor|
- Neonatal, moderately pigmented donor
- Neonatal, darkly pigmented donor
- Adult, lightly pigmented donor
|Medium 254 (500 ml)|
|Human melanocyte growth supplement (HMGS)|
Human melanocyte growth supplement-2 (HMGS-2)
|Dermal fibroblasts||- Neonatal|
|Medium 106 (500 ml)||Low-serum growth supplement (LSGS)|
|Dermal microvascular endothelial cells||- Neonatal|
|Medium 131 with attachment factor (500 ml)||Microvascular growth supplement (MVGS)|
Attachment factor (100 ml)
* The cells listed in Table 2 are also available as proliferating cultures available in their respective sections. All cells have tested negative for HIV-1, hepatitis B, hepatitis C, mycoplasmas, bacteria, yeast, and other fungi and are highly characterized.