TrypLE™ Testimonials from Users
 | Rouzbe R.Taghizadeh, PhD Chief Scientific Officer AuxoCell Laboratories, Inc. Amesbury, MA Who is Auxocell? AuxoCell Laboratories, Inc. is a leading stem cell therapeutic and regenerative medicine company. AuxoCell's primary research focus is to develop the enormous therapeutic potential of the primitive stem cells found in the Wharton's Jelly of the human umbilical cord. Through strategic partnerships with universities, stem cell centers, and research laboratories around the world, AuxoCell strives to become the world’s most respected authority on the Wharton’s Jelly derived stem cell. |
What’s your background and how did you get into cell therapy?
I obtained my PhD in stem cell bioengineering in 2006 from MIT. I was appointed Chief Scientific Officer of AuxoCell Laboratories, Inc., after completing my post-doctoral training from MIT and the Boston Biomedical Research Institute in 2008. Before that, I obtained my bachelors of science in chemical engineering from the University of Massachusetts, Amherst, in 2000. I have spent the past 10 years working to bring stem cell based therapies to clinical practice, with the aim of helping patients suffering from various diseases and cancers. I have worked extensively in adult stem cells, especially human hematopoietic stem cells
How has TrypLE™ made a difference in your work?
One of the main concerns while passing mesenchymal stem cells is to maintain normal karyotype and high viability of the cells. One morning I was preparing to passage six different flasks while working with Wharton's Jelly derived mesenchymal stem cells.
After a wash with HBSS, I added 1 mL of GIBCO® TrypLE™ Express to the last of the six flasks when suddenly the fire alarm went off in my building. Not knowing what had set off the fire alarm, I decided to attempt to save the cells by placing the flasks in the incubator to quickly detach the cells and subsequently dilute the TrypLE™ with HBSS. I placed the flasks in the incubator, but did not have the opportunity to dilute them with HBSS, since a fireman came to the cell culture room I was in and asked me to evacuate the building. So I had no choice but to leave the cells in the incubator in 1 mL of TrypLE .
It turned out that our generator room had flooded due to heavy rains overnight. It was not until 2:30 p.m. that we were allowed to re-enter the building. I took my flasks out of the incubator, prepared for the worst, but decided to proceed as normal in an attempt to save the cultures and was expecting significant cell lysis and poor cell viability.
When I looked at the cells under the microscope, to my pleasant surprise, the cells were intact (suspended in the TrypLE™), and more notably, viable—as viable as they would have been under normal conditions (~98%). I passaged the cells and the cultures continued propagating without any significant problems. Fortunately, I was using TrypLE™ to detach my cells (and not trypsin-EDTA) and was able to save my cultures, even though the cells were in TrypLE™ for 4+ hours. If I was using trypsin-EDTA, undoubtedly, the cells would have lysed in 20 minutes and I would have had to start my month-long experiment from the beginning.
After a wash with HBSS, I added 1 mL of GIBCO® TrypLE™ Express to the last of the six flasks when suddenly the fire alarm went off in my building. Not knowing what had set off the fire alarm, I decided to attempt to save the cells by placing the flasks in the incubator to quickly detach the cells and subsequently dilute the TrypLE™ with HBSS. I placed the flasks in the incubator, but did not have the opportunity to dilute them with HBSS, since a fireman came to the cell culture room I was in and asked me to evacuate the building. So I had no choice but to leave the cells in the incubator in 1 mL of TrypLE .
It turned out that our generator room had flooded due to heavy rains overnight. It was not until 2:30 p.m. that we were allowed to re-enter the building. I took my flasks out of the incubator, prepared for the worst, but decided to proceed as normal in an attempt to save the cultures and was expecting significant cell lysis and poor cell viability.
When I looked at the cells under the microscope, to my pleasant surprise, the cells were intact (suspended in the TrypLE™), and more notably, viable—as viable as they would have been under normal conditions (~98%). I passaged the cells and the cultures continued propagating without any significant problems. Fortunately, I was using TrypLE™ to detach my cells (and not trypsin-EDTA) and was able to save my cultures, even though the cells were in TrypLE™ for 4+ hours. If I was using trypsin-EDTA, undoubtedly, the cells would have lysed in 20 minutes and I would have had to start my month-long experiment from the beginning.
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