TrypLE™ - The Superior Replacement for Trypsin

TrypLE Express

TrypLE™ Express (1X), TrypLE™ Select (1X), and TrypLE™ Select (10X)

  • Gentle on cells - maintains cell health for reproducible results
  • Saves time - achieves faster dissociation for a wide variety of cells
  • Animal origin-free - pure, consistent and effective
  • Room-temperature stable - more convenient to use

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TrypLE™ Express - Uses significant economies of scale to make TrypLE easily accessible to all researchers

TrypLE™ Select (1X) - Manufactured on dedicated animal origin-free equipment

TrypLE™ Select (10X) - For fast dissociation of strongly adherent cells and manufactured on dedicated animal origin-free equipment

Additional Information:

Gentle and Convenient
TrypLE™ is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE™ is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol.

Stable at room temperature
TrypLE™ is room-temperature stable and ready to use when you need it. TrypLE™ cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

TrypLE™ Express

TrypLE™ Express proves that superior performance can be affordable.  Significant economies of scale make it even easier to replace trypsin with TrypLE™ Express.  For fast, effective cell dissociation that doesn’t harm your cells, use TrypLE™ Express.

TrypLE™ Select (1X)

TrypLE™ Select (1X) is manufactured on dedicated animal origin-free equipment in our state-of-the art facilities in Grand Island, New York.

Cell Line Release Times with TrypLE™ Select (1X)

  TrypLE™ Select
Cell LineMediaMean Time Required
for Cell Release (min)		Mean Viability (%)
293FDMEM + 5% FBS297
VEROVP-SFM5100
VEROEMEM + 5% FBS8100
VEROOptiPro798
CHO-K1CHO III A795

TrypLE™ Select (10X)

TrypLE™ Select (10X) can be used at full strength to quickly and gently dissociate strongly adherent cell types. TrypLE™ Select (10X) is also manufactured on dedicated animal origin-free equipment in our state-of-the art facilities in Grand Island, New York. 

Strongly Adherent Cell Line Release Times

  TrypLE™ Select 10xTrypLE™ Select
Strongly Adherent
Cell Lines		MediaMean Time Required
for Cell Release (min)		Mean
Viability (%)		Mean Time Required
for Cell Release (min)		Mean
Viability (%)
PK-15EMEM + 10% FBS11.7 ± 2.099.4 ± 0.32798
PK-15OptiProN/AN/A1199
MDCKOptiPro15.3 ± 0.898.7 ± 0.52898
MDBKDMEM + 5% FBS8.0 ± 1.398.8 ± 0.515100
A549DMEM + 10% FBS5.2 ± 0.399.1 ± 0.5998
MSCDMEM + 10% MSC FBS7.8 ± 1.298.7 ± 1.3N/AN/A
MSCStemPro MSC SFM8.7 ± 1.597.5 ± 0.1N/AN/A