Insect Cell Culture
Insect cell culture is a common choice for heterologous protein expression. For large scale production or basic research, insect cells are able to express large quantities of protein with complex post-translational modifications.
Which Insect Media is Right for You?
|Serum-Free Growth & Protein Expression||Highest Consistency & Protein Expression|
|Source origin||Animal||Serum Free||Animal Origin-Free|
|Lot-to-lot consistency||Low (FBS variability)||Medium (medium hydrolysate concentration)||High (low hydrolysate concentration)|
|Optimized to prevent foaming|
|Compatible insect cells||Sf9 & Sf21||Sf9 & Sf21||Sf9 & Sf21|
|Supported Cell Density (Sf9 cells/mL)||3 x 106 cells/mL||10 x 106 cells/mL||10–14 x 106 cells/mL|
|Packaging||100 mL, 500 mL, 10 x 1L (powder)||500 mL, 1000 mL, 5 L (bags), 10 L (bags), 20 L (bags)||500 mL, 1000 mL, 10 L (bags), 20 L (bags)|
|Price (500 mL)||$4 (powder), $36 (liquid)||$29–$37||$33–$40|
|Order Now||Order Now||Order Now|
Drosophila S2 Cell Culture
Drosophila S2 cells are used for heterologous protein expression in the Drosophila Expression System (DES). The S2 cell line was derived from a primary culture of late stage Drosophila melanogaster embryos (Schneider, 1972). This adaptable cell line grows without CO2, at room temperature, as a semi-adherent monolayer or suspension in Scheider's Drosophila Medium.
High Five™ Cell Culture
High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate, derived from the parental Trichoplusia ni cell line. They are optimized for the growth and maintenance of cells used with the Baculovirus expression vector system (BEVS) for adherent or suspension culture. Adapted to serum-free suspension culture in Gibco® Express Five® serum free medium.