Countess® New FeaturesOn-Screen Viability Validation | View Details |
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On-Screen Viability Validation
| Color-Coded Indicators After a count has been performed, you can retrieve a static image of the most current sample, which will have colored circles overlaid on each object, indicating whether the cell has been counted as live (blue circle) or dead (red circle), or if the cell has been excluded (black circle) from the count (Figure 1). Improved Accuracy This feature can be used after each count to ensure that cells have been properly counted–and to check the proper parameter setting when a new protocol is being used or a new cell type is being counted. | | ) (click to enlarge) Figure 1. The viability validation feature allows you to visualize how each cell was counted. |
Cell Gating
Gate by Size
This feature allows you to differentiate between cells of a mixed population based on cell size (Figure 2). The gate can be set prior to counting or after a count has been obtained to calculate the concentration of a particular population of cells. Post-count data manipulation can be done on the instrument and in the Countess® software with archived data using a computer.
More Control
Prior to counting, you simply set the cell size range to be included in the count (Figure 3). Once these parameters are applied, a count is performed and the instrument calculates the concentration of cells fitting within the parameter settings. Then you can view a graphical representation of the acquired information with the gate in place. If you are not satisfied with the count and concentration, you can reset the gate and the data will be recalculated without performing another count (Figure 4).
Sharpen Your Count
By setting a broad gate initially, then closing in on the desired population after counting, you can develop a protocol for a particular cell type.
This feature allows you to differentiate between cells of a mixed population based on cell size (Figure 2). The gate can be set prior to counting or after a count has been obtained to calculate the concentration of a particular population of cells. Post-count data manipulation can be done on the instrument and in the Countess® software with archived data using a computer.
More Control
Prior to counting, you simply set the cell size range to be included in the count (Figure 3). Once these parameters are applied, a count is performed and the instrument calculates the concentration of cells fitting within the parameter settings. Then you can view a graphical representation of the acquired information with the gate in place. If you are not satisfied with the count and concentration, you can reset the gate and the data will be recalculated without performing another count (Figure 4).
Sharpen Your Count
By setting a broad gate initially, then closing in on the desired population after counting, you can develop a protocol for a particular cell type.
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(click to enlarge) Figure 2. Counting results with gating information. | (click to enlarge) Figure 3. New Countess® Automated Cell Counter gating function. | (click to enlarge) Figure 4. Gating function can be reset after a count. |
Personal Protocol Maker
Personalized Protocol
The protocol maker is useful when a particular set of parameters is desired for a certain cell type or for use with mixed populations. This feature can save the set of parameters you program (Figure 5) as a protocol prior to counting a given sample. The protocol is then recalled and applied for use with the desired sample (Figure 6). Default settings can also be recalled and applied at the touch of a button.
The protocol maker is useful when a particular set of parameters is desired for a certain cell type or for use with mixed populations. This feature can save the set of parameters you program (Figure 5) as a protocol prior to counting a given sample. The protocol is then recalled and applied for use with the desired sample (Figure 6). Default settings can also be recalled and applied at the touch of a button.
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(click to enlarge) Figure 5. Countess® Automated Cell Counter can save protocols programmed by the user. | (click to enlarge) Figure 6. Protocol programmed by the user can be applied to subsequent counts. |
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