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Countess™ Cell Counter - Product Information


The Countess™ Automated Cell Counter offers easy and accurate cell counting and viability counts. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer.
Accuracy
  • Eliminates subjectivity of manual counting
  • Minimizes human error
  • Provides reproducible data

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Comprehensive Data
  • Counts live and dead cells
  • Provides viability
  • Measures average cell size

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Convenience
  • No setup or maintenance
  • Uses just 10μl of sample
  • Includes dilution calculator and data archiving

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Accuracy You Can Rely On

The Countess™ Automated Cell Counter uses trypan blue staining combined with a sophisticated image analysis algorithm to produce accurate cell and viability counts in just 30 seconds. The algorithm also measures average cell size of live, dead, and total cells to give you all the data you need to proceed with your experiments. The measurement range extends from 1 x 104 to 1 x 107 cells/ml, with an optimal range from 1 x 105 to 4 x 106 cells/ml, broader than that of a hemocytometer (view technical notes for more comparison data). The optimal cell size is between 5 µm to 60 µm (view validated cell lines). A handy dilution calculator even helps you determine how to prepare your sample for your next passage or experiment.

Countess Cell Counter
Countess Cell Counter
Figure 2—The comparison of the Countess™ cell counter to hemocytometer counts for a variety of cell types and shows no statistical difference in counts between the two methods.
Figure 3—Three experienced users counted a standard latex bead sample (1.0 x 106 ±10%) with a glass hemocytometer, disposable hemocytometer, and the Countess™ instrument.  Although the Countess™ instrument shows some variability, user variability is much greater for users counting with the glass hemocytometer.

No More Manual Counting

The Countess™ Automated Cell Counter eliminates the tedium and subjectivity of manual cell counting. Automated counting frees up your time, reduces eye strain, and minimizes subjective judgments that can lead to error. It takes 3 simple steps:

1. Mix 10 µl of sample with 10 µl of trypan blue, and pipet into Countess™ chamber slide
2.  Insert slide into the instrument
3. Press the "Count cells" button, results are displayed in 30 seconds


Simple Operation With Application Versatility

The Countess™ Automated Cell Counter is easy to use—just plug it in and go. The counter uses disposable chamber slides, so there’s no setup, cleaning, or maintenance required. Its automated cell counting allows you to perform experiments that are difficult or impossible using a hemocytometer. Dosage studies become a breeze. You can save all of your data on any USB drive for further analysis.