Collagen I, Rat Tail and Collagen I, Bovine for Cell Culture

Collagen Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.

GIBCO® offers Collagen I Rat Tail (in liquid form and on precoated tissue culture plates) and Collagen I Bovine (in liquid form) for 3D applications and as coating solution.
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GIBCO® Collagen I Rat Tail and Collagen I Bovine, Liquid Form

GIBCO® offers Collagen I Rat Tail and Collagen I Bovine at 5mg/ml concentration which gives the flexibility to dilute to lower concentrations.

Figure shows GIBCO® Collagen I and collagen from two other suppliers were prepared at the concentrations stated and allowed to solidify in individual wells of a 24-well plate. GIBCO® Collagen I forms a firm, clearer gel matrix for cell culture.

GIBCO® Collagen I, Coated Plates

  • Ready to use right from the package
  • Available in 6-well, 24-well, and 96-well formats
  • High-quality, consistent coating of rat tail collagen I  for culturing primary cells including hepatocytes and keratinocytes
  • Convenient room temperature storage

Key Applications for Collagen I

Collagen consists of three α-chains which form a triple helix, providing tensile strength to the extracelluar matrix (ECM). Collagen I is the most common fibrillar collagen and is found in skin, bone, tendons, and other connective tissues.

Collagen I plays key roles in cell biology and biomedical applications: 

  • Promotes attachment, proliferation, and differentiation in breast cancer cells1
  • Forms gland-like circular structures from embryonic stem cells2
  • Allows better representation of endometrial physiology and morphology in vitro, allowing for in vivo studies of endometrial cancer cell invasion3
  • Helps microvascular endothelial cells (HMVEC cells) adopt an in vitro spindle-shaped morphology and form solid cord-like assemblies4
  • Used for in vitro angiogenesis assays, is a barrier in cell invasion assays, and enables cell adhesion studies5-6
  • Supports a variety of cell types, including primary colon carcinoma, mouse liver progenitor, and rat pancreatic islet cells

References:

1.  Chandrasekaran, S., et al., J. Biol. Chem. 274(16):11408 (1999).
2.  Chen, S., R. et al. (2003) Stem Cells 21: 281-295.
3.  Park, D., et al. (2003) Cancer Letters 195: 185-192.
4.  Whelan. M.C., et al. (2003) J. Biol. Chem. Jan 3;278 (1):327-34
5.  Kokenyesi, R., K., et al. (2003) Gynecol Oncol 89: 60-72.
6.  Ritty,T. et al. (2003) J Ortho Res 21: 442-450
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