Mitogen Activated Protein Kinase Pathway

There are at least three distinct mitogen-activated protein kinase (MAPK) signaling modules which mediate extracellular signals into the nucleus to turn on the responsive genes in mammalian cells, including ERK, JNK, and p38 kinase. The MAPK signaling pathway is essential in regulating many cellular processes including inflammation, cell differentiation, cell proliferation and death. The role of MAPK pathway in cancer, immune disorders and neurodegenerative diseases has been well recognized.

Life Technologies offers antibodies, ELISAs, Luminex® multiplex assays and growth factors for key targets in the MAPK signaling cascade. 

 

Find MAPK signaling pathway primary antibodies, antibody pairs, ELISAs and Luminex® assays by protein target: 



Targets A–F

 

Targets G–L

 

Targets M–Z

    

Key MAPK Pathway Target

One key target, p38 MAPK, is activated in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), anisomycin, UV light, and growth factors. The activation of p38 MAPK is mediated by several upstream kinases including MAP kinase kinase 3 (MKK3), MAP kinase kinase 6 (MKK6) and MAP kinase kinase 4 (MKK4, also known as SEK1 and JNKK1). These kinases phosphorylate p38 MAPK at threonine 180 and tyrosine 182, resulting in p38 activation.

Western Blot Analysis

Western Blot with with Anti-p38MAPK Antibody

Western blot analysis of p38 MAPK [pTpY180/182] antibody peptide competition.

Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Prior to incubation with p38 MAPK [pTpY180/182] antibody, the immobilized membranes were incubated with the (lane 1) peptide immunogen, (lane 2) a generic phosphothreonine-containing peptide, (lane 3) a generic phosphotyrosine-containing peptide, (lane 4) the non-phosphorylated peptide corresponding to the phosphopeptide, (lane 5) no peptide, (lane 6) the phosphopeptide derived from the corresponding region of JNK1/2, and (lane 7) the phosphopeptide derived from the corresponding region of ERK1/2. The data show that only the phosphopeptide corresponding to p38 MAPK [pTpY180/182] blocks the antibody signal, thereby demonstrating the specificity of the phosphospecific antibody.

Immunohistochemistry

IHC with Anti-p38MAPK Antibody

Immunohistochemistry of human lung carinoma tissue labeled with rabbit anti-ERK1/2 [pT185/pY187].

FFPE human lung carcinoma tissue was labeled with rabbit anti-ERK1/2 [pT185/pY187] (5 µg/ml). Tissues were pretreated with EDTA, then detected with SuperPicTure™ Polymer DAB . Images were taken at 20x magnification. Note nuclear and cytoplasmic staining in tumor cells.

ELISA Quantification

ELISA Quantification of p38MAPK

ELISA quantification of total p38 MAPK and phosphorylated p38 MAPK [pTpY180/182] in vitro.

Recombinant p38 MAPK was phosphorylated in vitro using MKK6 enzyme. Non-phosphorylated p38 MAPK was used as control. The phosphorylated and non-phosphorylated p38 MAPK were analyzed with p38 MAPK [pTpY180/182] ELISA and p38 MAPK (Total) ELISA. Both forms of the p38 MAK protein were detected using the total ELISA whereas only the phosphorylated p38 MAPK protein was detected with the phosphospecific ELISA.

Find MAPK Protein Targets A–F

Target Abfinity™ Monoclonal Antibodies Recombinant Oligoclonal Antibodies Other Primary Antibodies** Antibody Pairs ELISA Proteins Luminex®
Assays
BDNF           PHC7074  LHC7071
BMP2 710022            
c-Met 700261     CHO0285 KHO0251    
c-Met (Soluble)       CHO0315 KHO2031    
c-Met [pYpYpY1230/34/35] 700139       KHO0281    
c-Raf [pS289/296/301]     A14039
(Rab)		        

 

Target Abfinity™ Monoclonal Antibodies Recombinant Oligoclonal Antibodies Other Primary Antibodies** Antibody Pairs ELISA Proteins Luminex®
Assays
CREB   710149          
CREB [pS133] 700129       KHO0241    
CREB Total         KHO0231    
Cytokine 30-plex (Human)             LHC6003
(Polystyrene)
LHC6003M
(Magnetic)
EGF      AHG0064
(Rab)		  

KHG0061
KHG0062

PHG0311
PHG0313
PHG0314
PHG0315

  LHG0061
EGFR      AHR5072
(Ms)		   KHR9061    
EGFR [pY1068] 701053       KHR9081   LHR9081
EGFR [pY1173]         KHR9071    
ERK1     138600
(Ms)		        
ERK1/2     44654G
(Rab)
136200
(Ms)		   KHO0081   LHO0231
ERK1/2 [pTpY185/187] 700012   44680G
(Rab)		   KHO0091   LHO0241
ERK1/2 [pTpY202/204]     368800
(Rab)		        
ERK5/BMK1     44688G
(Rab)		        
ERK5/BMK1 [pTpY218/220]     44612G
(Rab)		        
FAK 701094 710119          
FAK [pT925]     A14010
(Rab)		        
FGFbasic         KHG0021
KHG0022		 PHG0021
PHG0023
PHG0024
PHG0026
PHG0264		  LHG0021
**Rab=rabbit polyclonal IgG antibody. Ms=mouse monoclonal IgG antibody

Find MAPK Protein Targets G–L

Target Abfinity™ Monoclonal Antibodies Recombinant Oligoclonal Antibodies Other Primary Antibodies** Antibody Pairs ELISA Proteins Luminex®
Assays
IGF1-R   710065         LHO0491
IGF-1R [pYpY1135/1136]             LHO0501
IL-1α     AHC0912
(Ms)		   KAC1191 PHC0011
PHC0015
PHC0017		 LHC0811
IL-1ß        CHC1213 KHC0011
KHC0012
KHC0014		 PHC0811
PHC0815
PHC0816		 LHC0011

 

Target Abfinity™ Monoclonal Antibodies Recombinant Oligoclonal Antibodies Other Primary Antibodies** Antibody Pairs ELISA Proteins Luminex®
Assays
IL-2 701080   RHCIL220
(Rt)
AHC0722
(Ms)		  CHC1243 KHC0021
KAC1241		 PHC0021
PHC0023
PHC0026
PHC0027		 LHC0021
IL-6   710042 AHC0562
(Ms)		 CHC1263 KAC1261
KHC0061
KHC0061C
KHC0062
KHC0063
KHC0064
KHC0064C		 PHC0061
PHC0063
PHC0064
PHC0065
PHC0066		 LHC0061
LHC0063
IR             LHR9111
IR (B-subunit)         KHR9111    
IR [pY1158]         KHR9121    
IR [pY1328]         KHR9151    
IR [pY1334]         KHR9161    
IR [pY972]         KHR9141    
IR [pYpY1162/1163]         KHR9131   LHR9131
IRAK1 700025            
IRAK4 700026            
IκBα [pS32]         KHO0221    
JIP1 (N-Term)     345200
(Rab)		        
JIP1/2 (SH3)     345300
(Rab)		        
JNK   710075          
JNK1 701048   359800
(Ms)		        
JNK1/2     AHO1362
(Ms)		   KHO0121   LHO0071
JNK1/2 [pTpY183/185]     44682G
(Rab)		   KHO0131    
JNK2     AHO1362
(Ms)		        
**Ms=mouse monoclonal IgG antibody; Rab=rabbit polyclonal IgG antibody.  Rt=rat monoclonal IgG antibody

Find MAPK Protein Targets M–Z

 

Target Abfinity™ Monoclonal Antibodies Recombinant Oligoclonal Antibodies Other Primary Antibodies Antibody Pairs ELISA Proteins Luminex®
Assays
MAPKAPK2     AHO1242
(Ms)		        
MAPKAPK2 [pT334]     44516G
(Rab)		        
MEK1 [pS298]     44460G
(Rab)		        
MEK1 [pT286]     A14036
(Rab)		        

 

Target Abfinity™ Monoclonal Antibodies Recombinant Oligoclonal Antibodies Other Primary Antibodies Antibody Pairs ELISA Proteins Luminex®
Assays
MEK1 [pT292]     44458G
(Rab)		        
MEK1 [pT386]     44462G
(Rab)		        
MEK1/2 [pS222]     44452
(Rab)		        
MEK1/2 [pSpS218/222]/222/226     44454G
(Rab)		        
MEK2 [pT394]     44466G
(Rab)		        
MEK3     326900
(Ms)		        
MEK4 [pSpT257/261]     44474
(Rab)		        
MEK7 [pSpT271/275]     44478
(Rab)		        

    
       
MKK7     327000
(Ms)		        
NFκB         KHO0371    
p38 -Beta 2 MAPK     338700
(Ms)		        
p38 MAPK     331300
(Ms)		   KHO0061   LHO0031
p38 MAPK [pTpY180/182] 701057   44684G
(Rab)		 CHO0075 KHO0071    
p53 [pT81]     A13988
(Rab)		        
Pyk2 [pY402] 700632 710087          
RSK1     AHO1472
(Ms)		        
RSK1 [pSpS221/227]     44924G
(Rab)		        
Shc [pY239/240]     A13980
(Rab)		        
STAT2 701105 710135        
STAT3 [pY705]         KHO0481    
STAT5a         KHO0751    
STAT5a [pY694]         KHO0761    
STAT5b [pY699]         KHO5721    
STAT6 701110 710140        
STAT6 [pY641] 700247   44375M
(Ms)		      
TGFb1        CHC1683   PHG9202
PHG9203
PHG9204
PHG9211
PHG9214		  
TNFa   710162   CHC1753 KHC3011 PHC3011
PHC3013
PHC3015
PHC3016		 LHC3011
**Rab=rabbit polyclonal IgG antibody. Ms=mouse monoclonal IgG antibody

 

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.