Protein Labeling Kits

Protein Labeling Kits

Our Protein Labeling Kits provide a nearly effortless way to label 1 mg of IgG antibody with a fluorescent dye or hapten.

  • Simple–ready to use in 2 hours, requiring approximately 30 minutes actual hands-on time
  • Reliable–protein conjugates with stable attachment of fluorescent labels for subsequent use in fluorescence-based imaging or flow cytometry.
  • Convenient–Easiest method to label larger amounts of IgG antibody

Protein Labeling Kit Products

Label   Ex/Em†Cat. no.
Emission Color*		Flow Cytometry
Imaging

Alexa Fluor® 350346/442A10170
          ●
Order
Alexa Fluor® 488495/519A10235             ●          ●
Order
Alexa Fluor® 532532/553A10236
          ●
Order
Alexa Fluor® 546
556/573A10237
          ●
Order
Alexa Fluor® 555
555/565
A20174
          ●Order
Alexa Fluor® 568
578/603
A10238
          ●Order
Alexa Fluor® 594
590/617
A10239
          ●Order
Alexa Fluor® 633621/639
A20170             ●          ●Order
Alexa Fluor® 647
650/665
A20173             ●          ●Order
Alexa Fluor® 660663/690
A20171             ●          ●Order
Alexa Fluor® 680
679/702
A20172
          ●Order
Fluorescein
494/518
F10240             ●          ●Order
Oregon Green® 488
496/524
O10241             ●          ●Order
Pacific Blue™ dye
410/455
P30012             ●          ●Order
Pacific Orange™ dye
400/551
P30016             ●
Order
Texas Red® dye
595/615
T10244
          ●Order
Biotin
n/a
D20655
             ●          ●Order
* Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm.

How Protein Labeling Kits Work

The Protein Labeling Kits utilize an amine-reactive fluorophore or hapten to covalently attach the label to the IgG antibody.  Once formed, the covalent bond between the label and the protein is extremely stable and you are using the same chemistry we use to prepare our own primary and secondary conjugates. 

Simply add ~1 mg of purified IgG (in ~500 μL and free of amine-containing buffers such as Tris) to one of the vials containing a premeasured quantity of amine-reactive dye and a magnetic stir bar. Purification is accomplished on a gravity-feed size exclusion column supplied with the kit (Figure 1). The entire labeling and purification procedure can be completed in as little as 2 hours and everything needed to perform three conjugations is provided.
Protein Labeling Kits
Figure 1. Molecular Probes® Protein Labeling Kits are the simplest way to label 1 mg of IgG antibodies.
Each Protein Labeling Kit includes:
Material
Amount
Reactive dye
3 vials
Sodium bicarbonate
84 mg
Purification resin
~25 mL
10X Elution buffer
~25 mL
Purification column
3
Column funnel
3
Foam column holder
3
Disposable pipet
3
Collection tube, 4 ml
3


Product Information Sheet

Need to Label Other Antibody Amounts?

< 1-20 µg< 1-20 µg< 1-20 µg
Zenon® Antibody Labeling Kits

APEX™ Antibody Labeling Kits

Microscale Antibody Labeling Kits


< 1-20 µg< 1-20 µg
Monoclonal Antibody Labeling Kits

Small Animal In Vivo Imaging (SAIVI™) Antibody Labeling Kits