Protein Labeling Kits

Our Protein Labeling Kits provide a nearly effortless way to label 1 mg of IgG antibody with a fluorescent dye or hapten.

  • Simple–ready to use in 2 hours, requiring approximately 30 minutes actual hands-on time
  • Reliable–protein conjugates with stable attachment of fluorescent labels for subsequent use in fluorescence-based imaging or flow cytometry.
  • Convenient–Easiest method to label larger amounts of IgG antibody

Protein Labeling Kit Products

Label   Ex/Em†Cat. no.
Emission Color*		Flow CytometryImaging 
Alexa Fluor® 350346/442A10170           ●Order
Alexa Fluor® 488495/519A10235             ●          ●Order
Alexa Fluor® 532532/553A10236           ●Order
Alexa Fluor® 546556/573A10237           ●Order
Alexa Fluor® 555555/565A20174           ●Order
Alexa Fluor® 568578/603A10238           ●Order
Alexa Fluor® 594590/617A10239           ●Order
Alexa Fluor® 633621/639A20170             ●          ●Order
Alexa Fluor® 647650/665A20173             ●          ●Order
Alexa Fluor® 660663/690A20171             ●          ●Order
Alexa Fluor® 680679/702A20172           ●Order
Fluorescein494/518F10240             ●          ●Order
Oregon Green® 488496/524O10241             ●          ●Order
Pacific Blue™ dye410/455P30012             ●          ●Order
Pacific Orange™ dye400/551P30016             ● Order
Texas Red® dye595/615T10244           ●Order
Biotinn/aD20655             ●          ●Order
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. 

How Protein Labeling Kits Work

The Protein Labeling Kits utilize an amine-reactive fluorophore or hapten to covalently attach the label to the IgG antibody.  Once formed, the covalent bond between the label and the protein is extremely stable and you are using the same chemistry we use to prepare our own primary and secondary conjugates. 

Simply add ~1 mg of purified IgG (in ~500 μL and free of amine-containing buffers such as Tris) to one of the vials containing a premeasured quantity of amine-reactive dye and a magnetic stir bar. Purification is accomplished on a gravity-feed size exclusion column supplied with the kit (Figure 1). The entire labeling and purification procedure can be completed in as little as 2 hours and everything needed to perform three conjugations is provided.

Protein Labeling Kits


Figure 1. Molecular Probes® Protein Labeling Kits are the simplest way to label 1 mg of IgG antibodies.

 
Each Protein Labeling Kit includes:
MaterialAmount
Reactive dye3 vials
Sodium bicarbonate84 mg
Purification resin~25 mL
10X Elution buffer~25 mL
Purification column3
Column funnel3
Foam column holder3
Disposable pipet3
Collection tube, 4 ml3


Product Information Sheet

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Need to Label Other Antibody Amounts?

Zenon® Kits

< 1-20 µg		Microscale Kits

< 1-20 µg		Antibody Labeling kits

< 1-20 µg



 

Protein Labeling Kits

< 1-20 µg

 

SAIVI™ Kits

< 1-20 µg

 

APEX™ Antibody Labeling kits

10-20 µg




Learn More about APEX™ Antibody Labeling Kits
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