Monoclonal Antibody Labeling Technology
 | Molecular Probes® Monoclonal Antibody Labeling Kits label 100 µg IgG polyclonal and monoclonal antibodies with our superior Alexa Fluor®, Pacific Blue™, and Pacific Orange™ dyes.
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Monoclonal Antibody Labeling Kit Products
| Fluorophore | Ex/Em† | Cat. no. Emission Color* | Flow Cytometry | Imaging | |
|---|---|---|---|---|---|
| Alexa Fluor® 350 | 346/442 | A20180 | ● |  | |
| Alexa Fluor® 488 | 495/519 | A20181 | ● | ● | |
| Alexa Fluor® 532 | 532/553 | A20182 | ● | | |
| Alexa Fluor® 546 | 556/573 | A20183 | ● | | |
| Alexa Fluor® 555 | 555/565 | A20187 | ● | | |
| Alexa Fluor® 568 | 578/603 | A20184 | ● | | |
| Alexa Fluor® 594 | 590/617 | A20185 | ● | | |
| Alexa Fluor® 647 | 650/665 | A20186 | ● | ● | |
| Pacific Blue™ dye | 410/455 | P30013 | ● | ● | |
| Pacific Orange™ dye | 400/551 | P30014 | ● | | |
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. | |||||
How Monoclonal Antibody Labeling Kits Work
The Monoclonal Antibody Labeling Kits utilize an amine-reactive Alexa Fluor®, Pacific Blue™ or Pacific Orange™ fluorophore to covalently attach the label to the IgG antibody of interest. Once formed, the covalent bond between the label and the protein is extremely stable and you are using the same chemistry we use to prepare our own primary and secondary conjugates.
Unlike polyclonal antibodies and most other commercially available proteins, monoclonal antibodies are typically available only in small quantities. Simply adjust the protein concentration to ~1 mg/ml in the provided buffer, then add it to a vial of amine-reactive dye. Any stabilizing proteins (i.e., BSA) must be removed prior to the labeling reaction.
Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins (Figure 1). The entire labeling and purification procedure can be completed in as little as 90 minutes and everything needed to perform five conjugations is provided.
Unlike polyclonal antibodies and most other commercially available proteins, monoclonal antibodies are typically available only in small quantities. Simply adjust the protein concentration to ~1 mg/ml in the provided buffer, then add it to a vial of amine-reactive dye. Any stabilizing proteins (i.e., BSA) must be removed prior to the labeling reaction.
Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins (Figure 1). The entire labeling and purification procedure can be completed in as little as 90 minutes and everything needed to perform five conjugations is provided.
 Figure 1. Simply adjust the protein concentration to ~1 mg/ml in the provided buffer, then add it to a vial of amine-reactive dye. Purification is accomplished on a size exclusion spin column optimized for ≥40 kDa proteins. |
Product Information Sheet
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Need to Label Other Antibody Amounts?
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