Microscale Antibody Labeling Kits
Microscale Protein Labeling Kits conveniently label small amounts (20–100 μg) of purified protein with a bright, photostable Alexa Fluor® dye or the hapten, biotin-XX.
|Alexa Fluor® 488||495/519||A30006||●||●||Order|
|Alexa Fluor® 555||555/565||A30007||●||Order|
|Alexa Fluor® 594||590/617||A30008||●||Order|
|Alexa Fluor® 647||650/665||A30009||●||●||Order|
|* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm.|
How Microscale Antibody Labeling Kits Work
The Microscale Protein Labeling Kits use an amine-reactive fluorophore or biotin to covalently attach the label to the protein or IgG antibody. Once formed, the covalent bond between the label and the protein is extremely stable; you are using the same chemistry we use to prepare our own primary and secondary conjugates.
Unlike our other antibody labeling kits, these kits have been optimized for labeling proteins between 12 and 150 kDa; thus, they label other proteins besides IgG antibodies.
Any stabilizing proteins (i.e., BSA), must be removed prior to the labeling reaction. Convenient spin columns purify the labeled protein (Figure 1) with yields between 60 and 90%, depending on the molecular weight of the starting material. The entire labeling and purification procedure can be completed in as little as 2 hours—everything needed to perform three conjugations is provided.
Product Information Sheet
Need to Label Other Antibody Amounts?
|Zenon® Kits||Microscale Kits||Antibody Labeling kits|
Learn More about APEX™ Antibody Labeling Kits