Chemiluminescent ELISA Substrate
 | Chemiluminescent ELISA Kits—adaptable and sensitive for easy assay developmentWhether you’re performing standard ELISAs, receptor binding assays, or highly sensitive nucleic DNA probe capture assays, Chemiluminescent Alkaline Phosphatase ELISA kits are the adaptable solution to your assay needs.
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Rapid, Ultra-Sensitive Antigen Detection
Chemiluminescent Alkaline Phosphatase ELISA kits are available in four different formats and a convenient sampler format to enable the development of immunoassays with the sensitivity and throughput tailored to your research needs. Optimized Chemiluminescent Alkaline Phosphatase ELISA reagents provide rapid, quantifiable results and significantly extend the limits of detection beyond those of most colorimetric and isotopic methods. Kits are available with your choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrates and either Sapphire-II™ or Emerald-II™ luminescence enhancers. I-Block™ blocking reagent is also included to prevent nonspecific binding and reduce background.
Product Comparison
| Substrate/Enhancer Formulation | Signal Intensity (Relative Light Units) | Sensitivity | Time to Peak Signal @37ºC | Measurement Time Window |
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| Ready-to-Use CSPD® / Sapphire-II™ | 8,000 | ++++ | 7-10 min | 10 min to 10 hrs |
| Ready-to-Use CSPD® / Emerald-II™ | 40,000 | +++ | 7-10 min | 10 min to 10 hrs |
| Ready-to-Use CDP-Star® / Sapphire-II™ | 32,000 | +++ | 20-30 min | 30 minutes to 10 hrs |
| Ready-to-Use CDP-Star® / Emerald-II™ | 65,000 | +++ | 20-30 min | 30 minutes to 10 hrs |
Choose an Optimal Substrate/Enhancer Combination
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Schematic of chemiluminescence enhancement. |
The specific substrate and enhancer combination that will provide the highest sensitivity for each assay system can vary:
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