Novabright™ Chemiluminescent ELISA Substrate
Adaptable and sensitive for easy assay development
Whether you’re performing standard ELISAs, receptor binding assays, or highly sensitive nucleic DNA probe capture assays, NovaBright™ Chemiluminescent Alkaline Phosphatase ELISA kits are the adaptable solution to your assay needs.
Rapid, Ultra-Sensitive Antigen Detection
NovaBright™ Chemiluminescent Alkaline Phosphatase ELISA kits are available in four different formats and a convenient sampler format to enable the development of immunoassays with the sensitivity and throughput tailored to your research needs. Optimized NovaBright™ Chemiluminescent Alkaline Phosphatase ELISA reagents provide rapid, quantifiable results and significantly extend the limits of detection beyond those of most colorimetric and isotopic methods. Kits are available with your choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrates and either Sapphire-II™ or Emerald-II™ luminescence enhancers. I-Block™ blocking reagent is also included to prevent nonspecific binding and reduce background.
|Substrate/Enhancer Formulation||Signal Intensity (Relative Light Units) ||Sensitivity ||Time to Peak Signal |
|Measurement Time Window|
CSPD® / Sapphire-II™
|8,000 ||++++ ||7-10 min ||10 min to 10 hrs|
CSPD® / Emerald-II™
|40,000 ||+++ ||7-10 min ||10 min to 10 hrs|
CDP-Star® / Sapphire-II™
|32,000||+++||20-30 min||30 minutes to 10 hrs|
CDP-Star® / Emerald-II™
|65,000 ||+++ ||20-30 min ||30 minutes to 10 hrs|
Choose an Optimal Substrate/Enhancer Combination
Schematic of chemiluminescence enhancement.
The specific substrate and enhancer combination that will provide the highest sensitivity for each assay system can vary: