Apoptosis is morphologically and biochemically distinct from cell death by injury (necrosis). Since no single parameter fully defines cell death in all systems, it is often advantageous to use a multiparametric approach when studying apoptotic events and their temporal relationships. Two Molecular Probes® Vybrant® Apoptosis assay kits provide reagents to distinguish apoptotic cell populations from necrotic cell populations off the violet laser.
- Detection of multiple apoptotic parameters on the violet laser
- Increased multiplexing capabilities
- Available as stand-alone reagents or easy-to-use kits
|Annexin V, Pacific Blue™ Conjugate|
|Vybrant® Apoptosis Assay Kit #13 PO-PRO™-1/7-aminoactinomycin D|
|Vybrant® Apoptosis Assay Kit #14 Annexin V-Pacific Blue™|
Plot of calcein fluorescence collected with 530/30 nm bandpass filter vs. ethidium homodimer-1 fluorescence collected with 610/20 nm bandpass filter, showing clear separation of live and dead cells, as well as counting beads (C36950). A mixture of live and heat-killed Jurkat cells (human T-cell leukemia) was treated with the reagents in the LIVE/DEAD® Viability /Cytotoxicity Kit, counting beads were added, and the sample was analyzed by flow cytometry using 488 nm excitation.
|CountBright™ Absolute Counting Beads|
It contains a violet ratiometric membrane asymmetry probe, F2N12S, which can monitor changes in membrane asymmetry that occur during apoptosis through a change in the relative intensity of the two emission bands of the dye. Unlike annexins, this assay does not need any special buffers or wash steps and is less affected by cell membrane damage commonly found during the physical or chemical removal steps when assaying adherent cells, therefore providing better data quality.
- Accurate apoptotic analysis on trypsinized cells
- Simple 5 minute staining protocol
- Compatible with other blue excited apoptotic stains