Pacific Orange™ and Pacific Blue™ Dyes

Pacific Orange™ dye (emission maximum of 551 nm) is fully compatible with Pacific Blue™ dye (emission maximum of 455 nm), giving you the option of multiparametric analysis with the violet laser. The Pacific Orange™ and Pacific Blue™ product lines include direct antibody conjugates,  Zenon® Antibody Labeling kit,  as well as secondary antibody and streptavidin conjugates.

  • First dual-dye combination with the violet laser
  • Distinct emission spectra for minimal compensation
  • Frees other lasers for multicolor analysis
Pacific Orange™ Dyes Categories
Pacific Blue™ Dyes Categories
Violet Excitation for Multiplexing
Violet excitation for multiplexing. Human mononuclear cells were labeled with Pacific Blue™ anti-CD4 and Zenon® Pacific Orange™ anti-CD8 antibodies. Analysis was performed on the BD LSR™ II flow cytometer using 405 nm excitation and 450/50 nm and 585/42 nm emission filters. Inset shows spectra for Pacific Blue™ dye (blue lines) and Pacific Orange™ dye (orange lines).

Qdot® Nanocrystals

The extraordinary optical properties of Qdot® nanocrystals make them ideal labels for flow cytometry. Their extremely bright fluorescence emission makes them well suited for the detection of low-abundance extracellular proteins. Efficient optical excitation is possible using the 405 nm violet excitation light source. In addition, the narrow, symmetric emission profiles of Qdot® nanocrystal conjugates require substantially lower compensation, enabling better, more efficient multicolor assays using the violet laser. A wide range of markers directly conjugated with Qdot® nanocrystals are available, in addition to a host of streptavidin conjugates to detect biotinylated antibodies.

  • excited by 405 nm or 488 nm light to maximize use of violet or blue lasers
  • can be used in combination with existing organic dyes for increased flexibility
  • extremely bright for detection of low-abundance antigens
  • narrow emission spectra for minimal compensation with a single excitation source


Qdot® Nanocrystal Categories
Qdot® primary antibody conjugates
Multicolor analysis of CD3-positive and CD4-positive cell populations using Qdot® primary antibody conjugates. Human PBLs were stained with Qdot® 655 anti-CD3 and Qdot® 655 anti-CD4 antibodies. Lymphocytes were analyzed for fluorescence using violet diode laser excitation and 605/20 nm and 655/20 nm emission filters. Samples were run on a BD LSR™ II flow cytometer. Plots are gated on lymphocytes by side scatter/CD45. Axes are labeled with the bandpass filters used; plots are labeled with compensation values (arrows).

CountBright™ Absolute Counting Beads

CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolute cell counts using this single-platform method are more accurate and less complicated than cell concentration determined using multiple-platform testing. CountBright™ absolute counting beads can be used with any sample type, including no-wash/lysed whole blood.
CountBright Counting Beads
Determination of live and dead cells and absolute cell number
Plot of calcein fluorescence collected with 530/30 nm bandpass filter vs. ethidium homodimer-1 fluorescence collected with 610/20 nm bandpass filter, showing clear separation of live and dead cells, as well as counting beads (C36950). A mixture of live and heat-killed Jurkat cells (human T-cell leukemia) was treated with the reagents in the LIVE/DEAD® Viability /Cytotoxicity Kit, counting beads were added, and the sample was analyzed by flow cytometry using 488 nm excitation.

Product
CountBright™ Absolute Counting Beads