Pacific Green™ Dye
Pacific Green™ dye is a new and novel fluorophore in the family of Molecular Probes® dyes that can be excited by the violet laser and be detected in the second emission channel of any fl ow cytometer equipped with a violet laser. This approach allows you to expand the number of targets detected in your multicolor experiments.
Conjugates of Pacific Green™ dye can be multiplexed with the Pacific Blue™ dye and PacificOrange™ dye conjugates. All three fluorophores can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm.
Pacific Green™ dye offers:
• Increased flexibility in multicolor experiments
• Detected in the second emission channel (ex/em 411/510)
• Compatible with Pacific Blue™ dye and Pacific Orange™ dye
• Optimal for use on any fl ow cytometer equipped with a violet laser
Pacific Orange™ and Pacific Blue™ Dyes
Pacific Orange™ dye (emission maximum of 551 nm) is fully compatible with Pacific Blue™ dye (emission maximum of 455 nm), giving you the option of multiparametric analysis with the violet laser. The Pacific Orange™ and Pacific Blue™ product lines include direct antibody conjugates, Zenon® Antibody Labeling kit, as well as secondary antibody and streptavidin conjugates.
- First dual-dye combination with the violet laser
- Distinct emission spectra for minimal compensation
- Frees other lasers for multicolor analysis
Violet excitation for multiplexing. Human mononuclear cells were labeled with Pacific Blue™ anti-CD4 and Zenon® Pacific Orange™ anti-CD8 antibodies. Analysis was performed on the BD LSR™ II flow cytometer using 405 nm excitation and 450/50 nm and 585/42 nm emission filters. Inset shows spectra for Pacific Blue™ dye (blue lines) and Pacific Orange™ dye (orange lines).
- excited by 405 nm or 488 nm light to maximize use of violet or blue lasers
- can be used in combination with existing organic dyes for increased flexibility
- extremely bright for detection of low-abundance antigens
- narrow emission spectra for minimal compensation with a single excitation source
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CountBright™ Absolute Counting Beads
Determination of live and dead cells and absolute cell number
Plot of calcein fluorescence collected with 530/30 nm bandpass filter vs. ethidium homodimer-1 fluorescence collected with 610/20 nm bandpass filter, showing clear separation of live and dead cells, as well as counting beads (C36950). A mixture of live and heat-killed Jurkat cells (human T-cell leukemia) was treated with the reagents in the LIVE/DEAD® Viability /Cytotoxicity Kit, counting beads were added, and the sample was analyzed by flow cytometry using 488 nm excitation.