Reagent Selection Guide for Attune® Cyometer
The Attune® Acoustic Focusing Flow Cytometer comes equipped with 488 nm (20 mW) and a 405 nm (50 mW) or a 637 nm (50 mW) laser. This configuration allows up to six-color analysis in addition to forward and side scatter data collection and absolute cell counts, without the need for counting beads. Combined with the power of Molecular Probes® reagents, the Attune® Acoustic Focusing Cytometer is ideal for many cell analysis research protocols. |
Violet Excitation Laser
| Fluorescence Channel | |||
|---|---|---|---|
| Orange | Green | Blue | |
| Default Filter (nm) | 603/48 | 522/31 | 450/40 |
| Filter Range (nm) | 579–627 | 507–537 | 430–470 |
| Fluorescent Reagents | Pacific Orange™ Qdot® 605 | Qdot® 525 | Pacific Blue™ Alexa Fluor® 405 |
Blue Excitation Laser
| Fluorescence Channel | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Red | Orange | Green | |||||||
| Default Filter (nm) | 780/60 | 690/50 | 640LP | 575/24 | 530/30 | ||||
| Filter Range (nm) | 750-810 | 665-715 | >640 | 563-587 | 515-545 | ||||
| Fluorescent Reagents | PE-Cy®7 Qdot® 800 | PE-Cy® 5.5 PerCP Qdot® 705 TRI-COLOR® (PE-Cy®5) | PE-Alexa Fluor® 700 PE-Cy® 5.5 PE-Cy®7 PerCP Qdot® 655 Qdot® 705 Qdot® 800 TRI-COLOR® (PE-Cy®5) | PE JC-1 DiOC2(3) | Alexa Fluor® 488 Fluorescein CFSE JC-1 DiOC2(3) | ||||
Red Excitation Laser
| Fluorescence Channel | |||
|---|---|---|---|
| Near IR | Far Red | ||
| Default Filter (nm) | 780/60 | 660/20 | |
| Filter Range (nm) | 750-810 | 650-670 | |
| Fluorescent Reagents | APC-Alexa Fluor® 750 | Allyophycocyanin (APC)
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Quick Links | |||||
