Attune® Cytometer Specifications

System Attributes


Physical
Characteristics
Footprint (H x W x D): 16 in⁄40 cm x 23 in⁄58 cm x 17 in⁄43 cm
Weight: 64 lb⁄29 kg
Operating temperature: 15–30°C
Operating humidity: <80% noncondensing
Electrical requirements: 100–240 VAC, 50⁄60 Hz, <300 W

Optics


Excitation lasers: Vilot/Blue: 405 nm (50 mW) and 488 nm (20 mW) -- or -- Red/Blue: 638 nm (50 mW) and 488 nm (20 mW) lasers


Fluidics


Sample input rates: 1 sample⁄min
Sample flow rates: 25–1,000 µL⁄min
Calibrated delivery volumes for volumetric analysis
Standard and sensitive laser transit times
Sample loading volume: 100 µL–4 mL
Fluid storage within instrument with level sensing
Nominal fluid consumption of 1 L per day
Designed to accommodate 17 mm x 100 mm to 8.8 mm x 45 mm tubes

Note: The minimum sample analysis volume is 50 μL. However, the system requires that at least 220 μL is used to account for the analysis of volume + boost volume + safety volume.


Electronics		 


Data acquisition up to 20,000 events⁄sec
Resolution of at least 6 decades
User-adjustable PMT voltage
Flash upgradeable firmware


Performance

 


Height, area, and width measurements on all scatter and fluorescent channels
Particle size range: 1–45 µm
Sensitivity: MESF (Mean Equivalent Soluble Fluor) FITC <100 PE <50


Regulatory		 
Research use only; CE, UL, RoHS compliant

Attune® Cytometric Software
Includes a Minitower Windows® XP with 23-inch flat panel monitor
Software controls acquisition, analysis (width, height,
and area measurements), and instrument
Analysis features include overlay plots, backgates, and customized paramters
Up to 20 million events per file
No software licensing fees
Output file format: FCS 3.0
Live gating with automatic saving
User and administrator log-in

Optical Layout

 Detectors Filter
(center/bandpass)		  Designed to Detect
Forward Scatter (FSC)
and Side Scatter (SSC)		   Particles and cells using 405 nm laser
BL1 (off 488 laser) 530nm/30 nm FITC, Alexa Fluor® 488 dye, GFP, SYTOX® Green dye, Vybrant® DyeCycle™ Green stain, CellTrace™ CSFE, LIVE/DEAD® Fixable Green Dead Cell Stain
BL2 (off 488 laser) 575 nm/24 nm PE, Propidium Iodide, YFP, Vybrant® DyeCycle™ Orange stain
BL3 (off 488 laser) 640 nm longpass PerCP-Cy® 5.5 dye, PerCP, PE-Cy® 5 dye (TRI-COLOR® anti-body), PE-Cy® 5.5 dye, PE-Cy® 7, Vybrant® DyeCycle™ Ruby stain, SYTOX® AADvanced™ kit, LIVE/DEAD® Fixable Red Dead Cell Stain, Qdot® 655, Qdot® 705, and Qdot® 800 nanocrystal conjugates
VL1 (off 405 laser) 450 nm/40 nm Pacific Blue™ dye, Vybrant® DyeCycle™ Violet stain, LIVE/DEAD® Fixable Violet Dead Cell Stain, CellTrace™ Violet dye, FxCycle™ Violet dye, SYTOX® Blue dye, autofluorescence, Alexa Fluor® 405 dye
VL2 (off 405 laser) 522 nm/30 nm LIVE/DEAD® Fixable Aqua Dead Cell Stain, Qdot® 525 nanocrystal conjugates
VL3 (off 405 laser) 603 nm/48 nm Qdot® 605 nanocrystal conjugates, Pacific Orange™ dye, LIVE/DEAD® Fixable Yellow Dead Cell Stain

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