Attune® Cytometer Software
Software that performs to your specifications.
Attune® Cytometric Software is designed to provide powerful user-defined analysis using an intuitive interface for simplified experimental analysis. Build custom templates around specific research applications and saved for consistent experimental design. The Attune® Cytometric Software also offers the flexibility of unlimited copies and can be downloaded without licensing fees.
Watch the Software Demo Video
Which Software Package is Right for You?
- Download the tech note: Using Attune® data files in FlowJo® software
User-Defined Analysis, Flexible Setup
Templates can be built around specific research applications and saved for consistent experimental design. Compensation is automated or user-defined and can be set up using a compensation guide. Utilities such as quick-save, drag-and-drop, and copy-and-paste provide rapid manipulation with commonly used functions. Experiments can be easily set up with automated settings that can be completely customized and saved for future experiments.
No Software Licensing Fees
The Attune® Cytometric Software can be downloaded without licensing fees. The software can be added to any desktop or laptop computer at your institution without any additional costs. Results can be easily analyzed at your convenience at your own computer, allowing the next user to run experiments on the Attune® cytometer.
Attune® Software Features
|Instrument controls||Analysis setup|
|Visualization tools||File format compatibility|
Instrument Performance Tracking
The Attune® Cytometric Software provides automated baseline calculation and performance test functions with minimal user interactions. Performance tracking is facilitated through the reports function in the software.
Instrument performance tracking is critical for collecting and analyzing accurate experimental data. The Attune® Performance Tracking Beads are designed for use with the Attune® Cytometric Software to automatically characterize, track, and report performance measurements of the Attune® Acoustic Focusing Cytometer. The beads are used to define a baseline and conduct daily measurements of the cytometer. Vials contain enough beads for 50 measurements, and specific information for each lot is downloaded to the Attune® Cytometric Software prior to use.
Performance tracking for the Attune® cytometer includes a comprehensive set of procedures to monitor the daily performance of the instrument. The performance tracking process involves:
- Running performance-tracking beads
- Monitoring changes in the coefficient of variation and in the associated PMT voltage
- Tracking linearity of instrument performance
- Evaluating the detector sensitivity and background over time
- Automatic setting of laser delay
Instrument performance is affected by the lasers, optics, and fluidics of the instrument. For optimal instrument performance, it is critical to follow the manufacturer’s recommended schedules for maintenance.
Attune® Cytometric Software. The instrument control panel on the left side of the screen is used for experiment setup and control of laser selection, optics, and fluidics (flow rates). The main frame of this screen shows data analysis plots, while the right panel shows sub-file organization.
Levey-Jennings plot showing Attune® cytometer performance over time. This report is generated using data from performance-tracking beads.