Cell Cycle Analysis With the Attune® Flow Cytometer
 | Accurate cell cycle phase assignments rely on precise measurements of fluorescence intensity differences between multiple cell populations. The Attune® Acoustic Focusing Flow Cytometer is designed to deliver this precision regardless of the sample throughput rate. You no longer need to sacrifice throughput for sensitivity. |
Precision, Regardless of the Sample Throughput Rate
The figures below show the low-variability data obtained with the Attune® Acoustic Focusing Cytometer, even at high sample rates.
| Figure 3. Minimal data variation at high sample rates with the Attune® Acoustic Focusing Cytometer. Jurkat cells were fixed and stained with propidium iodide, treated with RNase, and analyzed at a concentration of 1 x 104 cells/mL on a high-end instrument that uses hydrodynamic focusing, and on the Attune® Acoustic Focusing Cytometer at different sample rates. The left peak in all graphcs reflects cells in G0/G1 phase, while the right peak reflects cells in G2/M phase. Note that as sample rates increase on the instrument that uses hydrodynamic focusing, the width of the G0/G1 and G2/M peaks increase, whereas for the Attune® cytometer the peaks are relatively stable, even at the highest sample rate of 1,000 µL/min. These results are quantitated in Table 1. | ||
Figure 4. Percent CV of G0/G1 at different flow rates on the Attune® cytometer and a competitor (C) at different smaple rates. Note the minimal change in variability (%CV) for the Attune® Acoustic Focusing Cytometer, even at a high sample rate. |
Table 1.
| Sample Rate | %G0/G1 | %S* | %G2/M | G0/G1 Ratio | %CV | |
|---|---|---|---|---|---|---|
| High-End Hydrodynamic Cytometer | 12 µL/min | 41.73 | 38.73 | 20.44 | 1.96 | 4.83 |
| 35 µL/min | 40.16 | 38.95 | 20.89 | 1.96 | 6.12 | |
| 60 µL/min | 44.60 | 26.17 | 29.23 | 1.90 | 7.76 | |
| Attune® Acoustic Focusing Cytometer | 25 µL/min | 45.20 | 40.29 | 14.51 | 1.94 | 3.22 |
| 100 µL/min | 42.81 | 38.72 | 18.74 | 1.94 | 3.17 | |
| 1,000 µL/min | 40.25 | 38.55 | 21.20 | 1.94 | 4.21 |
More Cell Types
In another cell cycle study, five cell types (listed below) were analyzed with the Attune® cytometer and with a high-end hydrodynamic focusing cytometer (ModFit analysis was performed in each case). The sample throughput rate of 25 µL/min was used for the analysis.
Additional cell types analyzed with the Attune® Flow Cytometer:
- Ramos B CellsKG-1
- KG-1 a Myeloblast Cells
- HL60 Promyeloblast Cells
- U266
- Myeloma Cells
- ST486 B Cells
- Download Cell Cycle Poster
- Download Cell Cycle Application Note
