Cell Viability Assays for Flow Cytometry

Cell Viability Assays for Flow Cytometry

Dead cells often give false positive results as they tend to bind nonspecifically to many reagents. Therefore, removing dead cells from your flow cytometry data is a critical step to ensure accurate results and analysis. Molecular Probes® cell viability assays for flow cytometry help researchers distinguish live and dead cell populations more accurately than only using forward and side scatter characteristics.

Nucleic Acid Dead Cell Stains

Nucleic Acid Dead Cell Stains  Molecular Probes® SYTOX® family of dead cell stains provide bright, accurate dead-cell discrimination in a variety of color options to meet any flow cytometry experimental design.

  • High–affinity nucleic acid stains for easy dead-cell discrimination
  • Multiple colors with minimal spectral overlap for expanded multicolor capabilities
  • No wash steps; just add, incubate, and analyze, for simplified protocols

Fixable Dead Cell Stains

LIVE/DEAD® Fixable Dead Cell Stains Molecular Probes® LIVE/DEAD® Fixable Dead Cell Stain Kits are designed to ensure accurate assessment of cell viability in samples after fixation and/or permeabilization.

  • Fixable—staining retained after fixation for simple live/dead analysis with intracellular phenotyping
  • Simple—fits into almost any staining and phenotyping protocols
  • Flexible—seven colors from which to choose for UV, 405, 488, or 633 lasers

Cell Viability Assays

LIVE/DEAD® Cell Viability Assays Life Technologies™ offers complete solutions for easy, sensitive determination of cell viability, cell vitality and compound cytotoxicity.

  • Avialable for animal, bacterial, yeast, and fungal cells
  • Compatible cell viability assays for flow cytometry, fluorescence imaging, and microplate assays
  • Multiple fluorescence emissions available from blue to far red

Cell Viabiliy Assays Selection Guide

Laser    Traditional Stains  Fixable Stains
UV
   SYTOX® Blue (450/50)* LIVE/DEAD® Fixable Blue Dead Cell Stain (350/40)*
405 nm
   SYTOX® Blue (350/40)* LIVE/DEAD® Fixable Violet Dead Cell Stain (450/40)*
LIVE/DEAD® Fixable Aqua Dead Cell Stain (530/50)*
LIVE/DEAD® Fixable Yellow Dead Cell Stain (585/42)*
488 nm
   SYTOX® Green (530/30)*
SYTOX® AADvanced™ stain (>650)*		 LIVE/DEAD® Fixable Green Dead Cell Stain (530/30)*
LIVE/DEAD® Fixable Red Dead Cell Stain (>650 or 600/20)*
532 nm
   SYTOX® Orange (585/42)*
SYTOX® AADvanced™ stain (>650)*		 LIVE/DEAD® Fixable Red Dead Cell Stain (>650  or red bandpass)*
561 nm
   LDS 751 (700/20)* LIVE/DEAD® Fixable Red Dead Cell Stain (>650  or red)*
633/5 nm
   SYTOX® Red (660/20)* LIVE/DEAD® Fixable Far Red Dead Cell Stain (660/20)*
LIVE/DEAD® Fixable Near IR Red Dead Cell Stain (780/60)*

*Recommended filters

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