Cell Proliferation Assays for Flow Cytometry
 | Cell Proliferation assays are an important set of fluorescence based tests that can monitor cell health and cell division by either evaluating DNA synthesis through thymidine incorporation or population doubling using dye dilution cell tracing stain. These assays provide a rich data set that can be analyzed to answer complex questions and provide direction for future experimentation.
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DNA Synthesis
 | Deliver accurate detection without harsh denaturation of newly synthesized DNA using Click iT® EDU assays, an excellent BrdU alternative.
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Dye Dilution Assays
 | Get uniform labeling, tight CV’s, non-toxic staining of live cells with CellTrace™ Cell Proliferation Kits.
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Antibodies for BrdU Detection
 | Selection of antibodies to thymidine analogs validated for flow cytometry.
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Cell Proliferation Assays for Flow Cytometry Selection Guide
| Laser | DNA synthesis | CellTrace™ kits | Antibodies for BrdU |
|---|---|---|---|
| 405 nm | Click-iT® EdU Pacific Blue™ Flow Cytometry Assay Kit (450/40)* Order |
CellTrace™ Violet Cell Proliferation Kit (450/40)* Order |
BrdU mouse monoclonal antibody (Clone MoBU-1) - Pacific Blue™ (450/40)* Order |
| 488 nm | Click-iT® EdU Alexa Fluor® 488 Flow Cytometry Assay Kit (530/30)* Order |
CellTrace™ CFSE Cell Proliferation Kit (530/30)* Order |
BrdU mouse monoclonal antibody (Clone MoBU-1) - Alexa Fluor® 488 conjugate (530/30)* Order |
| 633/5 nm | Click-iT® EdU Alexa Fluor® 647 Flow Cytometry Assay Kit (660/20)* Order |
CellTrace™ Far Red DDAO (660/20)* Order |
BrdU mouse monoclonal antibody (Clone MoBU-1) - Alexa Fluor® 647 conjugate (660/20)* Order |
*Recommended emission filter
