Cell Cycle Assays for Flow Cytometry
 | Flow cytometry, in conjunction with modeling algorithms, provides a powerful tool to assess cells in G0/G1 phase versus S phase, G2, or polyploidy. Life Technologies™ offers a series of fluorescent dyes to allow accurate cell cycle analysis in either live or fixed cell populations. |
Vybrant® DyeCycle™ Stains
 | The Vybrant® DyeCycle™ family of dyes offers robust fluorescent dyes for live cell cycle analysis with limited cytotoxicity using 405, 488, 532, or 633 nm excitation. The dyes have low cytotoxicity, allowing stained cells to be sorted and otherwise cultured or assessed with functional assays after staining.
|
FxCycle™ Stains
 | Analyzing cell cycle using FxCycle™ Violet, SYTOX® AADvanced™, or FxCycle™ Far Red stains allows for multiple color options for simplified fixed cell cycle analysis.
|
More Assays for Cell Cycle Analysis
Antibodies for Flow Cytometry
| Live Cell Cycle | Fixed Cell Cycle |
Cell Cycle Assays Selection Guide
| Laser | Live Cells | Fixed Cells | |
|---|---|---|---|
UV
|
Hoechst 33342 (450/50)* |
FxCycle™ Violet (450/50)* |
|
405 nm |
DyeCycle® Violet (440/40)* |
FxCycle™ Violet (450/50)* |
|
488 nm |
DyeCycle® Green (530/30)* |
SYTOX® AADvanced™ 695/40* |
|
532 nm |
DyeCycle® Orange (585/42)* |
SYTOX® AADvanced™ 695/40* |
|
561 nm |
— | SYTOX® AADvanced™ 695/40* |
|
633/5 nm |
DyeCycle® Ruby (695/40)* |
FxCycle™ Far Red (660/20)* |
*Recommended filters
