Multiparametric Apoptosis Assays for Flow Cytometry

Since there is no single parameter that defines programmed cell death, a combination of techniques is recommended for the reliable detection of apoptosis. Our multiparametric apoptosis assay kits are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single cell population. The kits are designed to detect various apoptotic parameters in a single flow cytometry assay, helping to dissect the early and middle stages of the apoptotic response.


Multiparametric apoptosis analysis using the Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis kit with PI. Jurkat cells (T-cell leukemia, human) were treated with 10 μM camptothecin for four hours (bottom panel) or left untreated as a control (top panel). Cells were then treated with the reagents in the kit and analyzed by flow cytometry. Note that the camptothecin-treated cells (bottom panel) have a higher percentage of apoptotic cells (indicated by “A”) than the basal level of apoptosis seen in the control cells (top panel). L = live cells, D = dead cells.
 Product Parameter 1
 Parameter 2Parameter 3Excitation (nm)
Cat. No.
Single Channel Annexin V/Dead Cell Apoptosis Kit, with Alexa Fluor® 488 annexin V and SYTOX® Green
 Membrane Asymmetry
 Necrosis
--
488
V13240
Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit, with PI
 Membrane Asymmetry
 Necrosis
--
488
V13241
FITC Annexin V/Dead Cell Apoptosis Kit, with PI
 Membrane Asymmetry
 Necrosis
--
488
V13242
Membrane Permeability/Dead Cell Apoptosis Kit, with YO-PRO®-1 and PI
 Membrane Asymmetry
 Necrosis
--
488
V13243
Chromatin Condensation Apoptosis Kit, with Hoechst 33342 and PI
 Chromatin Condensation
 Necrosis
--
UV/488
V13244
Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, with Hoechst 33342, YO-PRO®-1, and PI
 Chromatin Condensation
 Necrosis
--
UV/488
V23201
PE Annexin V/Dead Cell Apoptosis Kit, with SYTOX® Green
 Membrane Asymmetry
 Necrosis
--
488
V35112
APC Annexin V/Dead Cell Apoptosis Kit, with SYTOX® Green
 Membrane Asymmetry
 Necrosis
--
488/633
V35113
Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit, with C12 resazurin, APC annexin V, and SYTOX® Green
 Mitochondrial Health
 Membrane Asymmetry
Necrosis
488/633
V35114
Mitchondrial Membrane Potential/Annexin V Apoptosis Kit, with MitoTracker® Red and Alexa Fluor® 488 annexin V
Mitochondrial Health
Membrane Asymmetry
--
488
V35116
Violet Annexin V/Dead Cell Apoptosis Kit, with Pacific Blue™ annexin V/SYTOX® AADvanced™
Membrane Asymmetry
Necrosis
--
405/488
A35136
Violet Membrane Permeability/Dead Cell 1 Apoptosis Kit, with PO-PRO®-1 and 7-aminoactinomycin D
Membrane Asymmetry
Necrosis
--
405/488
V35123
Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant® DyeCycle™ Violet and SYTOX® AADvanced™
Chromatin Condensation
Necrosis
--
405/488
A35135

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