| | All EVOS® fluorescence cell imaging systems utilize LED light sources. That means you get high-intensity output over a short light path for the most efficient fluorophore excitation.- Shorter light path enables better detection of fluorescent signals
- Continuous illumination gives consistent results
- >50,000-hour bulb lifetime lowers your laboratory costs
- Adjustable light intensity reduces photobleaching
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Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.
Constant Illumination, Consistent Results
 | | Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS® systems have continuous light cube intensity, users can rely on consistent illumination and can compare results quantitatively from images acquired on different days. Figure 1. Stability comparison. |
Lower Long-term Maintenance Costs Using LED Bulbs
 | | The LED bulbs on the EVOS® systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (and 1,500 hours for a metal halide bulb). That means a 70–75% savings in the overall upkeep of your instrument. Figure 2. Illumination costs over time. |
Higher Transmission Efficiencies With Hard-Coated Filters
Hard-coated filter sets are more expensive, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission compared to traditional soft-coated filters. With the EVOS® system’s hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

Figure 3. Superior transmission efficiencies are observed using hard-coated filters on the EVOS instruments compared to soft-coated filters. Excitation filter (purple), emission filter (red), dichroic mirror (green); Alexa Fluor® 488 excitation (blue), Alexa Fluor® 488 emission (pink).