Figure 1—Comparison of EdU direct detection and BrdU secondary detection. Rats were treated with estradiol three days prior to a 2 hr pulse of EdU or BrdU administered intraperiotoneally. Proliferating cells, labeled red, were detected either with a click reaction using Alexa Fluor® 594 azide supplied in the Click-iT® EdU Alexa Fluor® 594 Imaging Kit (left panel) or with anti-BrdU antibody followed by Alexa Fluor® 594 goat anti–mouse IgG secondary antibody (right panel). Nuclei are stained with the blue-fluorescent counterstain Hoechst 33342.

		Figure 2—Imaging of mitotic arrest in A549 cells with nocodazole using the HCS Mitotic Index Kit. A549 cells were treated with 500 nM nocodazole for 24 hours at 37°C/5% CO2 and assayed using the HCS Mitotic Index Kit. Nuclear segmentation and DNA content measurements were done using DAPI or HCS NuclearMask™ Deep Red stain. At 500 nM, there was a strong increase in phospho-histone H3 staining indicative of mitotic cells. The images were quantitated using the Thermo Scientific Cellomics® ArrayScan® VTI platform.

		Figure 3—Use of the HCS DNA Damage Kit to determine genotoxicity and cytotoxicity in A549 cells. Cells were treated with 30 µM or 120 µM valinomycin for 24 hours at 37°C/5% CO2 and toxicity was assayed using the HCS DNA Damage Kit. Imaging and analysis was performed using a 10X objective and the Compartmental Analysis Bioapplication with the Thermo Scientific Cellomics® ArrayScan® VTI platform. At 30 µM valinomycin, cells were positive for  pH2AX, but not for Image-iT® DEAD Green™ viability stain indicating DNA damage, but not a compromise in plasma  membrane integrity. At 120 µM valinomycin, cells showed genotoxic and cytotoxic effects as demonstrated by the positive pH2AX and Image-iT® DEAD Green™ viability stain fluorescence.