SlowFade® Gold antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules for analysis. SlowFade® Gold antifade reagent does not cure over time so samples can be viewed immediately. SlowFade® reagents are intended for short-term preservation (3–4 weeks), and samples may degrade over longer periods.
SlowFade® Gold antifade reagent
- Inhibits photobleaching across the spectrum
- Non-setting formulation
- Available with or without DAPI
- Ready-to-use bench-top formulation
- Refractive index 1.42
SlowFade® Gold antifade reagent is best for immediate analysis and short-term storage. For long-term analysis and choose one of our ProLong® Gold antifade reagents.
SlowFade® Gold antifade performance
SlowFade® Gold antifade reagent outperforms all other commercially available antifade reagents because it preserves signal across the entire visible spectrum and causes little or no quenching of the initial fluorescent signal.
Tetrahymena pyriformis staining using mouse anti beta-tubulin monoclonal antibody.
Tetrahymena pyriformis were cultured with EdU, Click-iT® GalNAz glycoprotein labeling reagent, and InSpeck™ Blue (350/440) Intensity Calibration microspheres. Following fix/perm using Image-iT™ Fixation/Permeabilization kit, EdU-incorporated DNA was labeled with Alexa Fluor® 488 azide and GalNAz-incorporated cellular components with Alexa Fluor® 555 alkyne. Cilia were labeled with anti-beta-tubulin Ab and Alexa Fluor® 647 secondary Ab. Imaging followed mounting in SlowFade® Gold.
Protection across the spectrum
SlowFade® Gold antifade reagents offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.
Multiplex FISH detection using SlowFade® Gold antifade reagent, FISH Tag™ RNA and TSA™ kits.
In a whole mount Drosophila embryo, the expression of Krupple (magenta) and rhomboid (orange) were detected using FISH Tag™ RNA Kits with Alexa Fluor® 647 dye and Alexa Fluor® 555 dye or the Multicolor Kit. The expression of dpp was performed with a fluorescein-labeled RNA probe ChromaTide® fluorescein-12-UTP and detected with TSA™ Kit #2 with Alexa Fluor® 488 tyramide and Anti-fluorescein/Oregon Green®, rabbit IgG fraction, horseradish peroxidase conjugate.
Antifade reagents selection guide
|Format||5 x 2 mL||5 x 2 mL|
SlowFade® Gold performance across the spectrum
|Fluorescent dye|| |
Resistance to photobleaching*
|Alexa Fluor® 350||346/442||55%|
|Alexa Fluor® 488||495/519||90%|
|Alexa Fluor® 546||556/575||76%|
|Alexa Fluor® 555||555/565||89%|
|Alexa Fluor® 568||578/603||88%|
|Alexa Fluor® 594||590/617||92%|
|Alexa Fluor® 647||652/668||100%|
|Alexa Fluor® 680||679/702||96%|
|*Expressed as a percentage of initial fluorescence intensity remaining following 30 seconds of continuous illumination using a 40x/1.4NA objective and a 100-watt Hg-arc lamp as the light source.|
For added convenience and a reduction in the environmental impact associated with cold storage requirements, Life Technologies antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks.
|More information||Research tools|