SlowFade® Gold antifade performance SlowFade® Gold antifade reagent outperforms all other commercially available antifade reagents because it preserves signal across the entire visible spectrum and causes little or no quenching of the initial fluorescent signal. See performance data for SlowFade® Gold Antifade Reagent Tetrahymena pyriformis staining using mouse anti beta-tubulin monoclonal antibody. Tetrahymena pyriformis were cultured with EdU, Click-iT® GalNAz glycoprotein labeling reagent, and InSpeck™ Blue (350/440) Intensity Calibration microspheres. Following fix/perm using Image-iT™ Fixation/Permeabilization kit, EdU-incorporated DNA was labeled with Alexa Fluor® 488 azide and GalNAz-incorporated cellular components with Alexa Fluor® 555 alkyne. Cilia were labeled with anti-beta-tubulin Ab and Alexa Fluor® 647 secondary Ab. Imaging followed mounting in SlowFade® Gold.		Protection across the spectrum SlowFade® Gold antifade reagents offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. See dye compatibility for SlowFade® Gold Antifade Reagent Multiplex FISH detection using SlowFade® Gold antifade reagent, FISH Tag™ RNA and TSA™ kits. In a whole mount Drosophila embryo, the expression of Krupple (magenta) and rhomboid (orange) were detected using FISH Tag™ RNA Kits with Alexa Fluor® 647 dye and Alexa Fluor® 555 dye or the Multicolor Kit. The expression of dpp was performed with a fluorescein-labeled RNA probe ChromaTide® fluorescein-12-UTP and detected with TSA™ Kit #2 with Alexa Fluor® 488 tyramide and Anti-fluorescein/Oregon Green®, rabbit IgG fraction, horseradish peroxidase conjugate.