Image-iT™ FX Kits

All-in-One Kits for Fluorescence Imaging

The Image-iT™ FX Kits provide all of the dyes and reagents you need for optimal immunofluorescence imaging of fixed cells and tissue sections. Each kit includes:

  • Alexa Fluor® secondary antibody OR streptavidin conjugates for superior photostability and brightness
  • ProLong® Gold antifade reagent for reduced photobleaching
  • Image-iT™ FX signal enhancer for an improved signal-to-noise ratio


A pack of two CultureWell® chambered coverglasses (C37005) is also included for convenient processing. Each Image-iT™ FX Kit provides sufficient materials to perform 50–100 assays.

Alexa Fluor® Secondary Detection Conjugates

The Alexa Fluor® dye conjugates are, without exception, the best conjugates we have ever tested. With excellent brightness and photostability, these fluorescent conjugates of streptavidin, goat anti–mouse, and goat anti–rabbit IgG are superior fluorescent secondary reagents for imaging.

Alexa Fluor® Dye Conjugates

Alexa Fluor Dye Conjugates
 
Labeling cells with the bright and photostable Alexa Fluor® dye conjugates yields stunning, high-quality images.

ProLong® Gold Reagent - Ready-to-Use Antifade Reagent

ProLong® Gold antifade reagent is a new and improved version of the very effective ProLong® antifade reagent. It is premixed and ready to use — just add a drop to your preparation and mount. ProLong® Gold reagent outperforms most other commercially available antifade reagents, significantly reducing fluorophore photobleaching while causing little or no quenching of the fluorescence signal. This reagent offers excellent compatibility with a multitude of dyes and dye complexes, making it an especially valuable tool for multicolor applications. ProLong® Gold reagent cures within 24 hours, and the sample can be saved for months after mounting.

Learn More about ProLong® Gold antifade reagent

ProLong® Gold Antifade Reagent

 

ProLong gold antifade

 

ProLong® Gold antifade reagent (top) provides significant photobleaching protection compared to PBS alone (bottom) over a 20-second time series. Images were acquired at 5-second intervals.

Image-iT™ FX Signal Enhancer - Eliminate Background Staining

Image-iT™ FX signal enhancer dramatically improves the signal-to-noise ratio of immunolabeled cells and tissues, allowing you to clearly visualize targets that would normally be indistinguishable due to background fluorescence. Background staining typically seen with fluorescent dyes is largely eliminated when Image-iT™ FX signal enhancer is applied to fixed and permeabilized cells before staining (Table 1).

Treatment with Image-iT™ FX signal enhancer

Image-iT™ FX
 

Treatment with Image-iT™ FX signal enhancer (top) largely eliminates nonspecific fluorescent dye binding in cells.

Image-iT™ FX signal enhancer

 

Image-iT™ FX
 
 

Treatment with Image-iT™ FX signal enhancer (bottom) largely eliminates nonspecific fluorescent dye binding in a tissue section.

Table 1 - Fluorescent Dyes Successfully Tested with the Image-iT™ FX Signal Enhancer*

Alexa Fluor® 350
 Alexa Fluor® 633
 Cascade Blue®
Alexa Fluor® 405
 Alexa Fluor® 635
 Pacific Blue™
Alexa Fluor® 430
 Alexa Fluor® 647
 Fluorescein
Alexa Fluor® 488
 Alexa Fluor® 660
 Oregon Green® 488
Alexa Fluor® 532
 Alexa Fluor® 680
 Tetramethylrhodamine
Alexa Fluor® 546
 Alexa Fluor® 700
 Cy3
Alexa Fluor® 555
 Alexa Fluor® 750
 Rhodamine B
Alexa Fluor® 568
Rhodamine Red-X
Alexa Fluor® 594
Texas Red®, Texas Red-X
Alexa Fluor® 610

Cy5

* All dyes were conjugated to streptavidin and tested at 10 µg/mL.
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