Cell-Based Reporter Assays

We offer a number of reporter systems for cell-based assays.  GeneBLAzer® reporter assays use a β-lactamase gene combined with a FRET-enabled substrate to provide reliable and sensitive detection in cells.  Organelle Lights™ products using the brightest most stable fluorescent protein colors in Baculovirus-mediated  BacMAM™ delivery system puts our content or yours into cells with ease. TC- FlAsh™ and TC-ReAsh™ reagents are the smallest commercially available expression tags, offering two colors and convenience of Mammalian Gateway® cloning tools for live cell work.

Calcium Indicator Assays

Calcium Indicators 

Life Technologies offers the widest available selection of fluorescence-based indicators for calcium, including fura-2, indo-1, fluo-3, fluo-4, rhod-2, and the fluorescent protein-based Premo™ Cameleon Calcium Sensor.

 

Cell-Based ß-Lactamase Assays

Life Technologies offers GeneBLAzer® reporter assays which consists of  a β-lactamase gene combined with a FRET-enabled substrate to provide reliable and sensitive detection in cells. We also have fluorogenic substrates for β-lacatamase enzyme activity.

 

Fluorescent Protein Assays (GFP)

Fluorescent protein  Life Technologies offers novel technologies for cell-based fluorescent protein (FP) assays.  These include biosensors (Premo™ Cameleon indicator), organelle-specific assays (Organelle Lights™ and Cellular Lights™ constructs), a wide variety of vectors (Gateway®. TOPO® and Vivid Color™ reagents and kits) as well as fluorescent counterstaining reagents and kits. 

Tetracysteine (Lumio/FlAsH/ReAsH) Assays

The TC-FlAsH™/ReAsH expression tag-based fluorescence labeling technology was developed for live-cell imaging. It is ideal for protein localization or real-time protein production studies.