Oxidative Stress Detection

CellROX® Reagents are fluorogenic probes for measuring generalized oxidative stress in cells using conventional fluorescence microscopy, high content screening, microplate fluorometry, or flow cytometry. The dyes are nonfluorescent in a reduced state and fluoresce bright green, orange, or deep red upon oxidation. Some are formalin fixable for easy multiplexing with other fluorescent reagents including antibodies. Assays are simple and reliable with a sensitive and robust readout, and the reagent can be applied to cells in complete growth media. Life Technologies provides CellROX® dyes and conventional probes for oxidative stress measurement.

• Selection guide for generalized oxidative stress detection

Lipid peroxidation is the oxidative degradation of lipids. ROS are the major initiators of lipid peroxidation, and membrane-bound polyunsaturated fatty acids like arachidonic acid and linoleic acid are their major targets. The byproducts of lipid peroxidation cause direct damage to cell membranes. They also form protein adducts, resulting in cell and tissue damage. Lipid peroxidation is implicated in many human diseases including diabetes and cardiovascular disease. Life Technologies provides Molecular Probes® tools for live and fixed cells, with a choice of ratiometric or single-wavelength readout.

• Selection guide for lipid peroxidase detection

Superoxide, peroxyl radical, hydrogen peroxide, hydroxyl radical, and peroxynitrite are examples of ROS that react with nucleic acids, proteins, and lipids and result in cell and tissue damage. Certain ROS have been implicated in various human diseases including cancer, cardiovascular disease, neurodegenerative disease, and aging. Life Technologies provides selective Molecular Probes® reagents for a variety of reactive species targeted to the cytosol or mitochondria and detected using imaging, flow cytometry, or microplate analysis.

• Selection guide for selective ROS detection

Reduced glutathione, also known as GSH, is a major thiol bound to proteins. Protein thiols including GSH play an important role in determining the redox status of cells. Therefore, detection of GSH levels is a useful indication of redox potential and a cell’s ability to prevent oxidative stress. Life Technologies provides Molecular Probes® intracellular probes for the sensitive detection and localization of thiols.

• Selection guide for detection of glutatione levels