Cell Health Products Selection Guide
Reagents for counting cells and measuring cytotoxicity and proliferation are essential research and diagnostic tools. As life scientists, we understand the great diversity among live cells, their environments, and the physiological and morphological parameters that can be queried during the course of scientific investigation. Which assays researchers choose depend on what questions they are asking, the platform(s) to be used, and an evaluation of the advantages and limitations of the various assays that exist.
This guide highlights a few of the most popular and effective products Invitrogen has to offer for counting cells, determining cell viability, and assessing cell health by monitoring cytotoxic or proliferative effects.
This guide highlights a few of the most popular and effective products Invitrogen has to offer for counting cells, determining cell viability, and assessing cell health by monitoring cytotoxic or proliferative effects.
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| Countess® Automated Cell Counter | LIVE/DEAD® Fixable Stains | alamarBlue® for Cell Viability and Proliferation | CyQUANT® Proliferation Assays | Click-iT® EdU Proliferation Assays | |
| Answers the question: | How many live and dead cells are in my sample? What is my average cell size? | Which cells are dead and how can I discriminate between live and dead cells? | What is the metabolic health of the cells? | Have my cells multiplied? How many cells are present? | Which cells are actively proliferating? |
| Used primarily for: | Cell counting, viability measure, and average cell size calculation | Discrimination of live and dead cells in flow cytometry | Cell viability and cytotoxicity, indirect proliferation measurement | Cell counting via DNA content to assess cytotoxic or proliferative effects independent of cellular metabolism | Cell proliferation |
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| How it functions: | Counts live and dead cells via trypan blue membrane permeability; uses sophisticated algorithm to calculate cell number and percent viability | Allows post-fixation separation of live and dead cells from ~50X increase in fluorescent amine reactive dye intensity when bound to intracellular vs. surface proteins | Assays for cellular reducing environment where fluorescence or absorbance is proportional to the number of living cells | Quantitates relative count of cells in a population based on total DNA content by measuring intensity of dyes that fluoresce upon DNA binding | Measures rate of new DNA synthesis by EdU nucleoside analog incorporation into DNA with detection by a copper-catalyzed “click” reaction between an azide and an alkyne |
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