Fusion of a lysosome with the isolation membrane to form the autolysosome is the penultimate step of the autophagic pathway. Lysosomal membrane markers serve as tools for tracking fusion with the autophagosome prior to degradation of the autolysosome contents, whereas cell permeable LysoTracker® dyes target acidic organelles and are localized within the lysosomes in nanomolar concentrations. Both membrane proteins and Lysotracker dyes serve as effective tools for tracking lysosome behavior.
Autophagy describes the segregation and delivery of cytoplasmic cargo, including proteins and organelles, for degradation by hydrolytic enzymes through the lysosomal machinery. It is critical to the healthy functioning of cells and the failure of autophagy is a major reason for the accumulation of cell damage and aging.
Select Lysosomal Markers