Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments. Rhodamine phalloidin, which is made by conjugating phalloidin to the orange-fluorescent dye, tetramethylrhodamine (TRITC), is one of the most commonly used fluorescent phalloidin conjugates in the literature. As evidenced by over 1,500 citations (maintained in our in-house database), Molecular Probes® rhodamine phalloidin is the best choice for staining F-actin and is backed by the Molecular Probes® expertise in fluorescence technology.
| | Rhodamine phalloidin staining of F-actin in fibroblast cells. The actin cytoskeleton of a fixed and permabilized muntjac skin fibroblast was labeled with rhodamine phalloidin; the nucleus was stained with DAPI. The sample was mounted and imaged in SlowFade® Gold antifade reagent. | |
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