Mouse CD4+ Isolation

Which Mouse CD4+ T Cell Isolation Product is Right for You?

  Affordable & pure, flow compatible cells Highest purity for molecular assays High purity & physiological quality Untouched—most physiological relevant cells
Isolation method Positive isoation Depletion or positive isolation Positive isolation Negative isolation
Starting samples Any sample (whole blood, spleen, lymph node) Any sample (whole blood, spleen, lymph node) Any sample (whole blood, spleen, lymph node) Spleen, lymph node cells*
# Cells processed 2 x 109 cells 1-2 x 109 cells 2 x 109 cells 1 x 109 cells
Purity Higher
90-98%		 Higher
90-98%		 Higher
90-98%		 High
80-90%
Yield Standard
50-60%		 Highest
95-100%		 Standard
50-70%		 Standard
50-70%
Viability >90% >90% >90% >90%
Isolation time ~60min ~30 min ~90 min ~50 min
Isolation temperature cold cold release at room temperature cold
Applications Cellular assays
(cell culture, cell activation/proliferation, cytokine studies) Molecular:
isolation of proteins & nucleic acids		 Depletion of unwanted CD4+ T cells or
Positive isolation for molecular isolation of proteins & nucleic acids		 Cellular assays
(cell culture, cell activation/proliferation, cytokine studies)
Translational research
Molecular:
isolation of proteins & nucleic acids		 Cellular applications for physiological studies (functional studies, functional studies, adoptive transfer, flow analysis or further sorting)
Bead-free target cells Yes
(but antibody present)		 No Yes
(and antibody-free)		 Yes
Compatible with
flow cytometry
/flow sorting		 yes   yes yes
Shelf life from production date 2 years 3 years 3 years 3 years
Required materials Magnets Magnets Magnets Magnets
Price per 1x 107 cells** $3.16 $4.33 $4.33 + $1.43 $5.72
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* Lyse the red cells prior to use to improve the purity
** Additional 3% discount for academic accounts
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