Human B Cell Isolation
Which Human B Cell Isolation Product is Right for You?
| Highest purity for molecular assays | Pure flow compatible cells | Untouched - most physiological relevant cells | |||
|---|---|---|---|---|---|
| Isolation Method | Depletion or positive isolation | Positive isolation | Negative isolation | ||
| Starting samples | Any sample (whole blood, MNC, bone marrow) | Any sample (whole blood, MNC, bone marrow) | MNC* | ||
| # MNC processed
(mL whole blood processed) | 1-2 x 109 MNC 200 mL whole blood | 1-2 x 109 MNC 80 mL whole blood | 1 x 109 cells | ||
| Purity | Highest 98-100% | Highest 98-100% | High 90-95% | ||
| Yield | Highest 95-100% | Standard 50-70% | Standard 50-70% | ||
| Viability | >95% | >95% | >95% | ||
| Isolation Time | ~30 min | ~90 min | ~50 min | ||
| Isolation Temp. | Cold | Release at room temp. | Cold | ||
| Applications | Depletion of unwanted B cells or positive isolation for molecular applications, e.g. isolation of proteins & nucleic acids. | Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Translational research Molecular: isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells) | ||
| Bead-free Target Cells | No | Yes (and antibody-free) | Yes | ||
| Compatible with Flow Cytometry/Sorting | No | Yes | Yes | ||
| Shelf Life from Production Date | 3 years | 3 years | 2 years | ||
| Required Materials | Magnets | Magnets | Magnets | ||
| Price per 1x107 Cells | $1.78 | $1.78 + $1.07 | $6.72 | ||
| Price per mL Whole Blood** | $4.47 | $4.47 + $1.07 | -- | ||
| Order Now | Order Now | Order Now |
** Additional 3% discount for academic accounts
THIS IS FOR HUMAN RESEARCH - NOT FOR MOUSE
