Human Cell Isolation

Avoid these artifacts

See video of Miltenyi artifacts
in T-cells after 72 hr incubation

Human Cell Isolation

Gentle tube-based isolation of human cells from any sample and for any downstream assay, including flow cytometry.

Dynabeads® are available for :

Positive isolation and cell release (with FlowComp™, CELLection™ or DETACHaBEAD®)
Negative isolation of untouched cells
Depletion of unwanted cell types or positive cell isolation for molecular applications.

Cell type/ Subset	Product	Starting Sample	Isolation technique	Main benefits/ Applications
T cells
All subsets	Dynabeads® Untouched™ Human T Cells	MNC	Negative Isolation	Isolate untouched T cells by removing unwanted cells. This kit does not remove activated cells.
	Dynabeads® FlowComp™ Human CD45RA	MNC, Buffy coat/Whole blood, Pre-isolated CD4+/CD8+ T-cells	Positive Isolation	Isolate bead-free CD45RA+ cells. Combine with our positive or negative isolation kits for CD4 or CD8 prior to isolation, to obtain hightly pure naive CD4 cells (CD4+CD45RA+) or naive CD8 cells (CD8+CD45RA+).
CD2⁺ T cells	Dynabeads® CD2 Pan T	Whole blood, MNC	Depletion	Deplete CD2⁺ cells or positively isolate cells for molecular analysis.
CD3⁺ T cells	Dynabeads® FlowComp™ Human CD3	Whole blood, MNC	Positive Isolation	Isolate bead-free CD3⁺ T cells for use in any downstream cell-based assay.
	Dynabeads® CD3	Whole blood, MNC	Depletion	Deplete CD3⁺ cells or positively isolate cells for molecular analysis. For cell-based studies, cells will be detached from the beads by overnight incubation. Can also be used for short-term stimulation.
	T cell expansion products	Whole blood, MNC		Easily stimulate and expand T cells with these artificial antigen-presenting cells. Long shelf life, no need to maintain antigen-presenting cells in culture.
CD4⁺ T cells	Dynabeads® FlowComp™ Human CD4	Whole blood, MNC	Positive Isolation	Isolate bead-free CD4⁺ T cells for use in any downstream cell-based assay.
	Dynal® CD4 Positive Isolation Kit	Whole blood, MNC	Positive Isolation	Isolate bead- and antibody-free CD4⁺ T cells for use in any downstream cell-based assay.
	Dynabeads® CD4	Whole blood, MNC	Depletion	Deplete CD4⁺ T cells or positively isolate cells for molecular analysis.
	Dynabeads® Untouched™ Human CD4 T Cells	MNC	Negative Isolation	Isolate untouched CD4⁺ T cells by removing unwanted cells. This kit does not remove activated CD4⁺ cells.
	Dynal® T4 Quant Kit	Whole blood		Rapid and easy-to-use CD4⁺ T cell counting HIV monitoring. No need for sophisticated equipment or calibration curve.
CD8⁺ T cells	Dynabeds® FlowComp™ Human CD8	Whole blood, MNC	Positive Isolation	Isolate bead-free CD8⁺ T cells for use in any downstream cell-based assay.
	Dynal® CD8 Positive Isolation Kit	Whole blood, MNC	Positive Isolation	Isolate bead- and antibody-free CD8⁺ T cells for use in any downstream cell-based assay.
	Dynabeads® CD8	Whole blood, MNC	Depletion	Deplete CD8⁺ T cells or positively isolate cells for molecular analysis.
	Dynabeads® Untouched™ Human CD8 T Cells	MNC	Negative Isolation	Isolate untouched CD8⁺ T cells by removing unwanted cells. This kit does not remove activated CD8⁺ cells.
Regulatory T cells	Dynabeads® Regulatory CD4+ CD25+ T Cell Kit		Positive Isolation	Isolate bead- and antibody-free regulatory T cells with >80% FOXP3 expression and >95% purity (CD4⁺CD25⁺ expression). Isolated cells are compatible with any downstream application. See more results.
Regulatory T cells	Dynabeads® Human Treg Expander			Efficient expansion of regulatory T cells using Dynabeads® as artificial antigen-presenting cells
CD25⁺ T cells	Dynabeads® CD25	Whole blood, MNC	Depletion	Deplete or isolate activated CD25⁺ T cells with up-regulated CD25 antigen expression. Can also be used for other cells expressing CD25 such as B cells.
B cells
All subsets	Dynabeads® Untouched™ Human B Cells	MNC	Negative Isolation	Isolate untouched B cells by removing unwanted cells.
CD 19⁺ B cells	Dynabeads® CD19 Pan B	Whole blood, MNC	Depletion	Deplete or positively isolate CD19⁺ B cells. Combine with DETACHaBEAD® CD19 to obtain bead- free cells.
	DETACHaBEAD® CD19	Isolated CD19⁺ cells	Positive Isolation	Detach viable and phenotypically unaltered B cells from beads after positive isolation with Dynabeads® CD19.
Stem Cells
CD34+ Stem cells	Dynal® CD34 Progenitor Cell Selection System	Whole blood, MNC	Positive Isolation	Isolate bead- and antibody-free CD34⁺ cells for use in any downstream cell-based assay.
NK Cells
All Subsets	Dynabeads® Untouched™ Human NK Cells	MNC	Negative Isolation	Isolate untouched NK cells by removing unwanted cells.
Monocytes
CD 14⁺ cells	Dynabeads® CD14	Whole blood, MNC	Depletion	Deplete monocytes or positively isolate cells for molecular analysis.
	Dynal® Untouched™ Human Monocytes	MNC	Negative Isolation	Isolate untouched monocytes by removing unwanted cells.
	Dynabeads® FlowComp™ Human CD14	Whole blood, Buffy coat PBMC	Positive Isolation	Avoid possible artifacts by removing the cells from the beads after isolation and use the monocytes for any downstream assay, including monocyte-derived dendritic cells (Mo-DC) or cytokine release.
Dendritic Cells
All subsets	Dynabeads® Human DC Enrichment Kit	MNC	Negative Isolation	Reduce sorting time by typically 60-90% compared to sorting only. Reduce the need for fluorescent antibody for labeling of cells for your sorting by a factor of 6-14. This kit retains all DC subpopulations.
Endothelial Cells
CD31⁺ Cells	Dynabeads® CD31 Endothelial Cell	Whole blood, MNC	Depletion	Deplete CD31⁺ endothelial cells or positively isolate cells for molecular analysis.
Tumor Cells
Epithelial cells	CELLection™ Epithelial Enrich	Whole blood, MNC	Positive Isolation	Obtain up to 5 log enrichment of epithelial tumor cells. Enriched cells are viable, bead-free and suitable for immunocytochemical staining.
	Dynabeads® Epithelial Enrich	Whole blood, MNC	Depletion	Enrich epithelial tumor cells and lyse for mRNA isolation and RT-PCR amplification. Sensitivity of enrichment with RT-PCR can be as high as 1 tumor cell in 1 ml blood.
	Dynabeads® CD45	Whole blood, MNC	Depletion	Enrich epithelial tumor cells by depleting leucocytes. Two log enrichment of tumor cells in blood MNC is possible. Enriched tumor cells are viable, bead-free and suitable for immunocytochemical staining.
Leucocytes
CD 45⁺ cells	Dynabeads® CD45	Whole blood, MNC	Depletion	Deplete CD45⁺ cells or positively isolate cells for molecular analysis.
Granulocytes
CD15⁺ cells	Dynabeads® CD15	Whole blood, MNC	Depletion	Deplete CD15⁺ cells or positively isolate cells for molecular analysis.

Use Your Own Antibody (secondary products)

Our Streptavidin Dynabeads®, Secondary coated Dynabeads® and Surface activated Dynabeads® can be combined with your own antibody to isolate any cell type. Choose your preferred product in the below table or see some examples of applications.

Product	Starting sample	Benefits
Streptavidin Dynabeads®
Dynabeads® FlowComp™ Flexi NEW!	Single Cell Suspensions	Combine with your own antibody of choice to isolate any cell type from any species. This kit provides everything you need for labeling of antibodies and subsequent cell isolation. Beads are removed after isolation - you obtain pure and viable cells suitable for any downstream application.
CELLection™ Biotin Binder	Whole blood, MNC	Use with any biotinylated antibody to positively isolate cells from any species. Beads and antibodies are removed after isolation - you obtain pure and viable cells for use in any application. Read more about cell isolation using biotinylated antibodies.
Dynabeads® Biotin Binder	Whole blood, MNC	Isolate any cell from any species. This kit bind all biotinylated antibodies and is our most flexible secondary product for cell isolation or depletion. Read more about cell isolation using biotinylated antibodies.
Secondary coated Dynabeads®
Dynabeads® Sheep anti-Rat IgG	Whole blood, MNC	Isolate any cell type from any species except rat. For use with your primary IgG rat antibody of choice. Ideal for positive isolation, negative isolation or depletion of cells.
Dynabeads® Pan Mouse IgG	Whole blood, MNC	A flexible secondary product for cell isolation or depletion. It will bind all subclasses of mouse IgG and is Fc specific. Requires less antibody and has shown no cross reactivity to human, rat, hamster, goat, rabbit and guinea pig IgG.
CELLection™ Pan Mouse IgG	Whole blood, MNC	Use with any mouse IgG to positively isolate cells from any species EXCEPT mouse. Beads can be removed after isolation to leave pure and viable cells for use in any application.
Dynabeads® Sheep anti-Mouse IgG	Whole blood, MNC	Isolate any cell type from any species except mouse. For use with your primary mouse IgG1, IgG2a or IgG2b antibody of choice. Ideal for positive isolation, negative isolation or depletion of cells. Also available in clinical research grade as Dynabeads® ClinExVivo™ Sheep anti-Mouse IgG.
Dynabeads® Goat anti-Mouse IgG	Whole blood, MNC	Isolate any cell type from any species except mouse. For use with your primary IgG mouse antibody of choice. Ideal for positive isolation, negative isolation or depletion of cells.
Dynabeads® Rat anti-Mouse IgG1	Whole blood, MNC	Isolate any cell type from any species except mouse. For use with your primary mouse IgG1 antibody of choice. Ideal for positive isolation, negative isolation or depletion of cells.
Dynabeads® Rat anti-Mouse IgM	Whole blood, MNC	Isolate any cell type from any species except mouse. For use with your primary mouse IgM antibody of choice. Ideal for positive isolation, negative isolation or depletion of cells.
Dynabeads® Sheep anti-Rabbit IgG	Whole blood, MNC	Isolate any cell type from any species except rabbit. For use with your primary rabbit antibody of choice. This product can be used for eucaryotic cells.
Surface activated Dynabeads®
Dynabeads® M-450 Epoxy	Whole blood, MNC	Hydrophobic naked beads with glycidyl ether (epoxy) reactive groups. Ideal for coupling of antibodies for cell applications. Coupling reactions can be performed at neutral pH, with 24 hour incubation at room temperature. Also available in clinical research grade as Dynabeads® ClinExVivo™ Epoxy.
Dynabeads® M-450 Tosylactivated	Whole blood, MNC	Hydrophobic naked beads with p-toluenesulphonyl (tosyl) reactive groups. For coupling of antibodies for cell applications. Coupling reactions can be performed at neutral pH although high pH and incubation at 37°C will promote covalent binding.

MNC: Mononuclear Cells, click here for recommended sample preparation procedures


Related Pages Magnets Dynabeads® technology overview Mouse Cell Isolation Cancer Research Isolation of Other Cells Are your cell isolation results at risk?		Resources Isolation strategies Recommended sample preparation procedures References and application notes Request Invitrogen Cellular Analysis catalog

Avoid these artifacts

Human Cell Isolation

Use Your Own Antibody (secondary products)

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