Are Your Cell Isolation Results at Risk?

If you are using cell isolation technique that leaves iron or dextran either on the cell surface or in solution after isolation (e.g., biodegradable particles) you should read this page.
Recent publications have shown that when working with cells with biodegradable nanoparticles,
iron oxides and dextran have potentially cytotoxic/ immunogenic effects on cells.
Among the recently published articles on this topic are:

The Dynabeads® technology does not expose your cells to toxic substances such as iron oxides and dextran

Dynabeads Expose Cells
Each bead has an even dispersion of superparamagnetic material (γFe2O3 and Fe3O4) coated with a thin polymer shell to encase the magnetic material. This polymer bead shell protects your target from toxic exposure to iron oxide while ensuring an effective magnetic separation.
Eliminate uncertainty with Dynabeads® FlowComp™
As the immune system is designed to protect us from harmful substances, foreign structures such as iron oxides and dextran from this step increases the risk of unwanted reactions and decreases the certainty of your end result. Utilizing the proven Dynabeads® technology, Dynabeads® FlowComp™ ensures reliable and reproducible results, and also allows you to move seamlessly into any downstream cell-based application.


With Dynabeads® FlowComp™ you're guaranteed:
  • UNPARALELLED REPRODUCIBILTY
    During the development of Dynabeads® FlowComp™ products, we have performed extensive investigations on a large number of blood donors/mice to ensure that we deliver products that give minimal performance variations (e.g. see figure)

  • HIGH VIABILITY AND RECOVERY
    It is desirable to minimize the number of apoptotic cells in your sample, as they can affect your data and results, i.e. a lower overall recovery. While a column-based method for Mouse CD4 isolation may give as little as 49% recovery, Dynabeads® FlowComp™ Mouse CD4+ Isolation Kit yields 83% total recovery of CD4+ T cells (live, non-apoptotic cells).

  • HIGH PURITY
    Cell isolation with Dynabeads® FlowComp™ has shown up to 99% purity of isolated cells.