Cell Isolation with Dynabeads® Technology
Gentle and tube-based magnetic separation with Dynabeads® is the technology of choice when you want to isolate high yields of pure, viable and functional cells. Your cells are not exposed to the stress of going through a dense column, and there's no risk of your results being affected by the isolation method. |
Selection Guides for Cell Isolation Products:
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Cell Separation Products
| Human Cell Separation | Mouse Cell Separation | Secondary Cell Separation |
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Positive Cell Isolation
In positive isolation, Dynabeads® bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells,
| Bead-free Cells Directly after cell separation, the beads can easily be released and removed using the innovative Dynabeads® FlowComp™ technology. Other Dynabeads® release systems include the CELLection™, and the DETACHaBEAD™ technologies. | ) click image to see FlowComp™ workflow |
Negative Cell Isolation
In negative isolation, Dynabeads® are used to deplete all the unwanted cells using the supplied antibody mix. Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.
- Learn More about Negative Cell Separation
Should you want to refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary-coated Dynabeads® you can create your own negative isolation kit for isolation of any cells from any species.
Depletion
In depletion, cell-specific Dynabeads® are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant - still containing all your wanted cells - for downstream assays. Click for more info on: Dynabeads® for human cell depletion and mouse cell depletion.
You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.
Avoid These Artifacts in T-Cells
Watch the video of Miltenyi artifacts in T-cells after 72 hr incubation. Tell us what you think and see what your colleagues are saying. |
Why Wait For Drips?
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Resources
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