Human Dendritic Cell Enrichment
Reduce your sorting time and increase your recovery of human dendritic cells
Dendritic cells comprise an essential component of the immune system. These cells are crucial in the initiation of antigen specific immune responses, as antigen presenting cells (APCs) to naïve T cells. In the past years, several dendritic cell subsets have been identified. As the various dendritic cell subsets exert different effects in adaptive immune responses, the ability to retain and isolate all subsets of dendritic cells has become critical to understanding immune responses.
Increase your recovery of subsets of dendritic cells by using Dynabeads® Human DC Enrichment kit in combination with flow sorting. T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes are depleted from mononuclear cells (MNC) to leave a bead- and antibody free population of cells highly enriched for dendritic cells.
You can expect an 80-100% recovery of the Lin- CD4+ cells, untouched and viable. Enriched dendritic cells can be further isolated to both myeloid and plasmacytoid dendritic cell subpopulation by flow sorting.
With this gentle depletion of non-dendritic cells, your sorting time will be reduced by a factor of typically 10-15 compared to sorting only. The additional benefit is that you can reduce the need of fluorescent antibody for labeling of cells. Pre-enrichment thus saves both money and time!
For successful dendritic cell isolation the Dynabeads® Human DC Enrichment kit should be used with the magnets MPC-L, MPC-50 or MPC-15.
Figure1. The principle of the human dendritic cell enrichment process