Green Fluorescent Protein (GFP) Antibodies

Checkout our ABfinity™ Recombinant Rabbit Monoclonal anti-GFP antibody–raised against full-length GFP and suitable for multiple applications, including western blots, immunopreciptation, ELISA, flow cytometry, immunohistochemistry, and immunocytochemistry. It is a simple choice for your GFP detection needs.

  • High quality and versatility
  • One anti-GFP antibody, multiple applications
GFP-expressing Lanthascreen Erk2 Figure 1—Anti-GFP Abfinity™ recombinant rabbit monoclonal antibody for flow cytometry applications. GFP-expressing Lanthascreen™ Erk2 A375 cells were stained with 0.5 µg anti-GFP Abfinity™ recombinant rabbit monoclonal antibody  followed by Alexa Fluor® 647 labeled goat anti–rabbit IgG. The black line represents signal obtained when the anti-GFP antibody is present. The red line represents cells pre-incubated with recombinant GFP. The gray line (control) represents cells stained without the anti-GFP antibody present.

U2OS cells expressing Organelle Lights ER-GFP

Figure 2—Anti-GFP Abfinity™ recombinant rabbit monoclonal antibody for immunocytochemistry applications. U20S cells expressing Organelle Lights™ ER-GFP (A) were analyzed using the anti-GFP Abfinity™ recombinant rabbit monoclonal antibody. Cells were fixed in 4% formaldehyde, permeabalized in 0.1% Triton X-100/PBS, and blocked in 1% BSA. The cells were then incubated with primary antibody at 1 µg/mL, followed by a Alexa Fluor® 647 goat anti-rabbit IgG (B). The nuclei were stained with Hoechst 33342. The merged signal (yellow, C) indicates co-localization of GFP fluorescence and the detection antibody.

Other anti-GFP Antibody Offerings

  • Rabbit polyclonal anti-GFP—raised against anti-GFP, rabbit serum isolated directly from Aequorea victoria and is available as a complete antiserum or as an anti-GFP, rabbit IgG fraction purified by ion-exchange chromatography.

  • Chicken anti-GFP antibody—raised against GFP isolated directly from Aequorea victoria and the IgY fraction is purified by affinity purification. Since the chicken lacks a classic “Fc” domain, it does not bind to mammalian IgG Fc receptors, resulting in lower backgrounds during immunostaining protocols. The chicken IgY is also antigenically different from the mammalian IgG, allowing you to perform double immunostaining experiments using antibodies from multiple species.

  • Anti-GFP antibody conjugates (including Alexa Fluor® dye conjugates)—use our popular anti-GFP rabbit polyclonal antibody to avoid an additional secondary antibody detection step. Great care has been taken to ensure that our conjugates do not contain free label that would contribute to higher backgrounds.

All anti-GFP antibodies are suitable for the detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis; the rabbit antibodies are also useful for immunoprecipitation.

Unconjugated anti-GFP Antibodies

Product Name HostCat. no. Application† Type 
anti-GFP Abfinity™ recombinant rabbit monoclonal antibody RabbitG10362 ELISA, IP, IHC, WB, Flow mAb order
anti-GFP, rabbit serum RabbitA6455 IP, IHC, WB Serum order
anti-GFP, chicken IgY fractionChickenA10262 ICC, WB IgY fraction order
anti-GFP, rabbit IgG fraction RabbitA11122 IP, IHC, WB IgG fractionorder

† IP = immunoprecipitation, IHC = immunohistochemistry, WB = western blot, ICC = immunocytochemistry, Flow =  Flow cytometry.

Direct Conjugates of anti-GFP Antibodies

Product Name Host Cat. no. Label  
anti-GFP, rabbit IgG fraction Rabbit A21311 Alexa Fluor® 488 order
anti-GFP, rabbit IgG fraction Rabbit A21312 Alexa Fluor® 594 order
anti-GFP, rabbit IgG fraction Rabbit A31851 Alexa Fluor® 555 order
anti-GFP, rabbit IgG fraction Rabbit A31852 Alexa Fluor® 647 order
anti-GFP, rabbit IgG fraction Rabbit A10259 Biotin-XX order
anti-GFP, rabbit IgG fraction Rabbit A10260 HRP order
anti-GFP, chicken IgY fraction Chicken A10263 Biotin-XX order
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