Antibody Purification
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CaptureSelect™ Antibody Toolbox® products offer:
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CaptureSelect™ Antibody Toolbox® products are designed to enhance the success of your antibody purification processes. The products offer specific advantages over purification using Proteins A, G, and L, including unique target specificity and simplified elution protocols. Each product has defined target specificity for distinct antibody types and regions, minimizing the need for you to test multiple media products to determine the optimal choice.
Single-step purification of many antibody formats
Many kinds of antibody formats can be purified with one of our Antibody Toolbox® products. Unlike the common bacterial coat protein matrices, Proteins A, G, and L, CaptureSelect™ ligands are developed with defined specificity and high avidity to the target protein of interest. This results in affinity chromatography resins well suited for single-step protein purification.
CaptureSelect™ Antibody Toolbox® resins for biomolecule purification
| IgG all sublasses | Characteristics |
|---|---|
| CaptureSelect™ IgG-CH1 (resin) | Suitable for purification of human IgG and all Fab fragments |
| CaptureSelect™ IgG-CH1 (pre-packed column) | Suitable for purification of human IgG and all Fab fragments |
| CaptureSelect™ IgG-Fc (Hu) | Specific for human IgG, recognizing all four subclasses |
| CaptureSelect™ IgG-Fc (ms) | Designed to purify IgG from multiple species by high affinity binding to the Fc region of IgG’s |
| Light chains | Characteristics |
|---|---|
| CaptureSelect™ LC-kappa (Hu) | To purify all human kappa Ig light chain containing products (previously known as CaptureSelect™ Fab kappa) |
| CaptureSelect™ LC-kappa (mur) | Murine Ig binder recognizing IgG, IgM, IgA, IgD, IgE, Fab fragments, Poly Ig from mouse, rat and guinea pig |
| CaptureSelect™ LC-lambda (Hu) | To purify all human lambda Ig light chain containing products (previously known as CaptureSelect™ Fab lambda) |
| CaptureSelect™ LC-lambda (ung) | Suitable for Ig’s, Fab fragments and free light chains from ungulates (bovine, horse, donkey, sheep, goat, pig) |
| CaptureSelect™ LC-lambda (mouse) | Mouse Ig binder recognizing IgG, IgM, IgA, IgD, IgE, Fab fragments containing a lambda light chain |
| CaptureSelect™ LC-lambda (rat) | Ig binder recognizing IgG, IgM, IgA, IgD, IgE, Fab fragments containing a lambda light chain from Rat and Guinea Pig |
| Isotype specific | Characteristics |
|---|---|
| CaptureSelect™ IgM | IgM resin suitable for human, mouse and rat IgM's |
| CaptureSelect™ IgA | For purification of human IgA, IgA-dimers and secretory IgA (sIgA) |
| CaptureSelect™ IgA-CH1 (Hu) | For purification of human IgA, IgA-dimers and secretory IgA (sIgA) and Fab fragments thereof |
| IgG subclass specific | Characteristics |
|---|---|
| CaptureSelect™ IgG4 (Hu) | Specific for human IgG4 without any crossbinding to other subclasses or species |
| CaptureSelect™ IgG1 (Hu) | Specific for human IgG1 without any crossbinding to other subclasses or species |
| CaptureSelect™ IgG3 (Hu) | Specific for human IgG3 without any crossbinding to other subclasses or species |
*A selection of the species that have been tested. Refer to the product sheet for more detailed information.
Hu = human, Pr = primates, Bo = Bovine, Hr = Horse, Gt = goat, Sh = sheep, Do = donkey, Gp = guinea pig, Mo = mouse, Rb = rabbit, Ch = chicken, ms = multi species, mur = murine, ung = ungulate
Purchase Antibody Toolbox® resinsPharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.
