Downstream Purification for Microbial Protein Production

High Performance Resins for Microbial Expression Systems

When you need high performance and high throughput on a large scale, POROS® Bulk Chromatography Resins are the industry standard for process-scale bioseparations. Microbial protein production is an important platform for the production of biotherapeutic proteins. POROS has many purifcation tools in both analytical and process tools for the large scale purification of this large scale expression system.  

Find out more about our POROS® resins.

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Benefits of a Revised Approach
to Anion Exchange Flow-Through
Polish Chromatography

The New POROS® XS Cation Exchange Resin Delivers a Combination of High Capacity + High Resolution + High Salt Tolerance

Today’s high titer biotherapeutic processes create a host of downstream purification challenges and bottlenecks. Traditional high-high capacity resins fail to achieve high resolution in purification.  The need for a resin that delivers a combination of high capacity, high resolution, and high salt tolerance can lead to increase process efficiency and help enable a faster time to market for the product.

  • Dynamic binding capacity for proteins, greater than 100 mg/mL at 5% breakthrough
  • High resolution using optimized 50 μm particle size for improved impurity clearance
  • Robust salt tolerance that maintains dynamic binding capacity to 150 mM NaCl (15 mS/cm)

Products:

POROS® XS Cation Exchange Resins

State-of-The-Art Manufacturing Capability

Life Technologies manufactures POROS® resins from approved raw materials to finished product using advanced, proprietary processes that make us unique in this industry. A 30,000 square foot, state-of-the-art chemical facility, in Bedford, Massachusetts, is dedicated to the production of all the POROS® chromatography resins. Quality testing is performed at all stages to ensure excellent lot-to-lot consistency. POROS® chromatography resins are manufactured under ISO 9001:2008 and ISO 13485:2003 standards. The facility and quality systems are routinely audited by regulated biopharmaceutical companies.

  • POROS® XS 50 µm Bulk Resin
    A cation exchange resin that delivers the combination of high capacity, high resolution, and high salt tolerance can lead to increased process efficiency and help enable a faster time to market for the product.
  • POROS® HQ 50 µm Bulk Resin
    Strong Anion Echange Resin available for low pressure applications, including large scale downstream bioprocessing. POROS® HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography® media designed for the separation and purification of biomolecules.
  • POROS® PI 50 µm Bulk Resin
    Weak Anion Echange Resin for low pressure applications, including large scale downstream bioprocessing. POROS® PI is based on a polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography® media designed for the separation and purification of biomolecules.
  • GoPure™ Pre-packed Columns
    A pre-packed column solution for biopharmaceutical manufacturers in early phase product development, the GoPure™ Pre-packed Chromatography Column from Applied Biosystems® combines connect-and-go convenience with high performance POROS® resin.

 

  • POROS® Heparin 50 µm Bulk Resin
    Heparin affinity media for process scale separation of biomolecules with sepcific Heparin binding affinity, such as growth factors and other serum components, including coagulation factors and nucleotide binding proteins.
  • POROS® R1 50 µm Bulk Resin
    Reversed-Phase Resin for low pressure applications, including large scale downstream bioprocessing. POROS® R1 is a 4000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
  • POROS® R2 50 µm Bulk Resin
    Reversed-Phase Resin for low pressure applications, including large scale downstream bioprocessing. POROS® R2 is a 2000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.