Downstream Purification for Antibody Production

High Performance Resins for Monoclonal Antibody Production

As cell culture technology improvements continue to result in monoclonal antibody expression level increases, the protein manufacturing throughput bottleneck has shifted to downstream purification. The bottleneck of the process exists at the capture chromatography step, where it can take days to process a single reactor harvest due to flow rate and capacity limitations of existing rPA affinity medias. Chromatography medias with high dynamic binding capacity and high throughput capability are required to minimize this process bottleneck.

POROS® Chromatography resins with its optimized pore structure results in high dynamic binding capacities being maintained over a large flow rate range and range of bed heights thereby improving the operational throughput and process flexibility.

Find out more about our POROS® resins.

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Benefits of a Revised Approach
to Anion Exchange Flow-Through
Polish Chromatography

The New POROS® XS Cation Exchange Resin Delivers a Combination of High Capacity + High Resolution + High Salt Tolerance


  

Today’s high titer biotherapeutic processes create a host of downstream purification challenges and bottlenecks. Traditional high-high capacity resins fail to achieve high resolution in purification.  The need for a resin that delivers a combination of high capacity, high resolution, and high salt tolerance can lead to increase process efficiency and help enable a faster time to market for the product.

  • Dynamic binding capacity for proteins, greater than 100 mg/mL at 5% breakthrough
  • High resolution using optimized 50 μm particle size for improved impurity clearance
  • Robust salt tolerance that maintains dynamic binding capacity to 150 mM NaCl (15 mS/cm)

Products:

POROS® XS Cation Exchange Resins

Applied Biosystems® GoPure™ Prepacked Chromatography Columns Offer Convenient, Flexible, and Efficient Purification of Antibodies

 

Ideal for pilot-scale chromatography unit operations, Applied Biosystems® GoPure™ Prepacked Chromatography Columns from Life Technologies deliver equivalent chromatographic performance
and greater convenience at a cost comparable to packing a traditional column.

  • Connect-and-go convenience speeds up process setup and facility turnaround time
  • Prequalified columns simplify process scale-up or modeling
  • Flexible column design can be tailored to process requirements

Products:

GoPure™ Pre-Packed Columns

High Performance Resin for Analytical and Lab-Scale Separations

POROS Analytical Columns, with 10 and 20 micron particle size resins, deliver excellent resolution at high speed for both analytical and lab-scale preparative applications.

POROS Protein A Analytical Columns are the industry standard for monitoring monoclonal antibody titer and yield from cell culture supernatant. POROS® Analytical Chromatography columns can be operated on any standard High Performance Liquid Chromatography (HPLC) or medium pressure chromatography system.

  • POROS® XS 50 µm Bulk Resin
    A cation exchange resin that delivers the combination of high capacity, high resolution, and high salt tolerance can lead to increased process efficiency and help enable a faster time to market for the product.
  • POROS® HQ 50 µm Bulk Resin
    Strong Anion Echange Resin available for low pressure applications, including large scale downstream bioprocessing. POROS® HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography® media designed for the separation and purification of biomolecules.
  • POROS® PI 50 µm Bulk Resin
    Weak Anion Echange Resin for low pressure applications, including large scale downstream bioprocessing. POROS® PI is based on a polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography® media designed for the separation and purification of biomolecules.
  • POROS® MabCapture™ A Resin
    New design in Protein A chromatography resin technology combines improvements made in bead and chemistry technology. These advances provide a monoclonal antibody purification media that not only demonstrates the highest dynamic binding performance at low flow rates but also one that can maintain superior binding capacity at high linear velocities where other chromatography resin products cannot operate
  • POROS® Heparin 50 µm Bulk Resin
    Heparin affinity media for process scale separation of biomolecules with sepcific Heparin binding affinity, such as growth factors and other serum components, including coagulation factors and nucleotide binding proteins.
  • POROS® R1 50 µm Bulk Resin
    Reversed-Phase Resin for low pressure applications, including large scale downstream bioprocessing. POROS® R1 is a 4000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
  • POROS® R2 50 µm Bulk Resin
    Reversed-Phase Resin for low pressure applications, including large scale downstream bioprocessing. POROS® R2 is a 2000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
  • POROS® A 20 µm Columns
    Prepacked Protein A Affinity Column is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
  • POROS® G 20 µm Columns
    Prepacked Protein G Affinity Column is based on an immobilized recombinant Protein G functional group designed for high throughput purification of antibodies.

 

 

POROS® A and G and
CaptureSelect® Affinity Columns

Learn More About POROS® Chromatography