Analytical Chromatography

High Performance Resin for Analytical and Lab-Scale Separations

Analytical ChromatographyPOROS® Analytical Chromatography Columns, available in 10 and 20 micron particle size, enable rapid separation for biomolecules for both analytical and lab-scale preparative applications.

POROS® Protein A Analytical Columns are the industry standard for monitoring monoclonal antibody titer and yield from cell culture supernatant.  The addition of Poros® CaptureSelect™ Analytical Columns expand the advantages of high-speed, high-performance quantitation to IgM, Fab fragments and fusion proteins.

POROS® Analytical Chromatography columns can be operated on any standard High Performance Liquid Chromatography (HPLC) or medium pressure chromatography system

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Analytical ChromatographyAnalytical ChromatographyAnalytical Chromatography
POROS® CaptureSelect™
Affinity Columns
POROS® A20

POROS® A and G and
CaptureSelect™

State-of-the-Art Manufacturing Capability

Life Technologies manufactures POROS® resins from approved raw materials to finished product using advanced, proprietary processes that make us unique in this industry. A 30,000 square foot, state-of-the-art chemical facility, in Bedford, Massachusetts, is dedicated to the production of all the POROS® chromatography resins. Quality testing is performed at all stages to ensure excellent lot-to-lot consistency. POROS® chromatography resins are manufactured under ISO 9001:2008 and ISO 13485:2003 standards. The facility and quality systems are routinely audited by regulated biopharmaceutical companies.

Affinity Resin

  • POROS® A 20 µm Columns
    Prepacked Protein A Affinity Column is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
  • POROS® G 20 µm Columns
    Prepacked Protein G Affinity Column is based on an immobilized recombinant Protein G functional group designed for high throughput purification of antibodies.
  • POROS® CaptureSelect™ 20 µm Columns
    Prepacked POROS® CaptureSelect™ Affinity Columns are based on an immobilized single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae  and are designed for high throughput purification of IgG, IgM, Fab Fragments, HSA or IgG Fc fusion proteins.

Activated Affinity Resin

  • POROS® AL 20 µm Columns
    Prepacked Immobilized Aldehyde Affinity Column is based on an aldehyde functional group designed for the immobilization of proteins through free amine groups.
  • POROS® EP 20 µm Columns
    Prepacked Immobilized Epoxide Affinity Column is based on an epoxide functional group designed for the immobilization of proteins through free amine groups.
  • POROS® OH 20 µm Columns
    Prepacked Immobilized Hydroxyl Affinity Column is based on an hydroxylated functional group designed for the immobilization of proteins through activation with tresyl-chloride, divinyl-sulphone or cyanogen bromide.

  • POROS® HQ 10 & 20 µm Columns
    Prepacked Strong Anion Exchange Column is based on a quarternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography® media designed for the separation and purification of biomolecules.
  • POROS® PI 20 µm Columns
    Prepacked Weak Anion Exchange Column is based on a polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography® media designed for the separation and purification of biomolecules.
  • POROS® HS 20 µm Columns
    Prepacked Strong Cation Exchange Column is a high capacity, strong cation exchange Perfusion Chromatography® media suitable for the separation and purification of biomolecules.
  • POROS® S 10 & 20 µm Columns
    Prepacked Strong Cation Exchange Column is a strong cation exchange Perfusion Chromatography® media, suitable for the separation and purification of biomolecules.

Metal Chelate

  • POROS®  MC 20 µm Columns
    Prepacked Immobilized Metal Affinity Column is based on a Imido-diacetate functional group designed for the purifiction of proteins that form co-ordination complexes via metal sites and specific amino acids, such as histidine tagged fusion proteins.

Reversed-Phase

  • POROS® R2 10 & 20 µm Columns
    Pre-packed Reversed-Phase Column is a 2000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
  • POROS® R1 10 & 20 µm Columns
    Pre-packed Reversed-Phase Column is a 4000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
  • POROS® oligo R3 30 µm Columns
    Pre-packed Reversed-Phase Column is a 300 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
  • Proszyme Immobilized Enzyme Cartridges
    POROS® 20 µm media with immobilized Pepsin or Trypsin providing complete, automated digestions in 1-5 minutes, with high sample recovery

 

  • POROS® HP2 20 µm Columns
    Prepacked Hydrophobic Interaction Chromatography Column is a high density phenyl, Perfusion Chromatography® media suitable for the separation and purification of proteins.
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