Connect with Bioproduction



Adventitious Virus Testing

MMV and Vesi virus contamination is a potential threat to mammalian cell culture manufacturing processes. Multiple cell culture manufacturing facilities have been negatively impacted over the past 20 years following contamination with these viruses. The ViralSEQ™ Detection System is an integrated DNA purification and real-time PCR–based test for rapid, sensitive detection of MMV and Vesi virus from a wide range of sample types.
  • Detection of all known Mouse Minute Virus (MMV) and Vesi virus strains
  • Proven TaqMan® technology for highly sensitive detection down to 10 DNA copies per reaction (viral particles equivalent to 0.002 TCID50)
  • Specific, no cross-reactivity with unrelated DNA
  • Discriminatory positive/extraction DNA control to eliminate possibility of false positive test results from accidental cross-contamination with control DNA
  • Use with Prep SEQ™ 1-2-3 Nucleic Acid Extraction Kit, which is optimized for highly efficient DNA recovery
  • Rapid time-to-results, typically in less than 4 hours

View workflow

 

High Sensitivity and Broad Dynamic Range.

The ViralSEQ® Vesivirus Assay can detect from 106 down to 10 copies of in vitro–transcribed Vesivirus RNA per reaction.
Please join us:

Are you able to detect mycoplasma, vesivirus, and mouse minute virus in under 4 hours? 

Watch our free webinar to find out more:

Cell culture contaminant detection

Vesivirus Detection Assay − Early Access

 
  • Detection of all available Vesivirus genomes
  • Rapid time-to-results in less than 5 hours
  • TaqMan®-based Real-Time PCR assay
  • Demonstrated sensitivity and specificity
  • Proprietary optimized sample preparation for high RNA recovery

Mouse Minute Virus (MMV) Detection Assay

  • Rapid time-to-results in less than 5 hours
  • TaqMan®-based Real-Time PCR assay
  • Demonstrated sensitivity and specificity
  • Proprietary optimized sample preparation for high RNA recovery

Step-by-Step Guide to Virus Detection Systems Products

Extract nucleic acid (DNA for MMV or RNA for Vesi virus) using the PrepSEQ 1-2-3 Nucleic Acid Extraction kit. The DNA (for MMV detection) you purify in this step will be used as your template for Real-Time PCR,while the RNA (for Vesivirus detection) will be used as a template in a one-step Reverse Transcription Real-Time PCR.

Products

View ordering information

Bioproduction Resources

Have questions about Gibco® media, POROS® chromatography resins, Applied Biosystems® SEQ rapid contaminant tests, or our Cell Therapy products?  Click below to access product brochures, training videos, mobile apps, and more.

  

Looking for Help?

Request a custom media quote
Sales Support
Services Support
Join us at an Event (Tradeshows, Seminars, Webinars, etc)
Schedule a Site Tour
Select Language