Host Cell Residual DNA Quantitation
The use of biologically derived drugs, in which host cell lines are used to express biological product, has increased dramatically in recent years. This has created a need to quantify residual host cell DNA during manufacturing and in final products.
Watch our free webinar to learn more about Host cell protein and residual dna.
Step-by-Step Guide to Host Cell Residual DNA Quantitation Products
The PrepSEQ™ Residual DNA Sample Preparation Kit and protocols are optimized to enable highly efficient DNA recovery from typical biopharmaceutical purification process samples. Consistent performance has been demonstrated across a wide variety of complex test sample matrices. We have also verified recovery of CHO DNA spiked into the following matrices:
- Acetate (20 mM) pH 3.5
- Acetate (500 mM) pH 3.5
- HCl (12 mM) pH 3.0
- Glycine (20 mM) pH 2.0
- Glycine (500 mM) pH 2.0
- Acetate/NaCl (300 mM/0.5 M) pH 4.5
- MES/NaCl (300 mM/0.5 M) pH 6.5
- Bis (300 mM) pH 6.5; MES (300 mM) pH 6.0
- Phosphate (300 mM) pH 5.0
- Tris (50 mM) pH 8.0
Achieve efficiencies through automation
Automation of the sample preparation procedure on the MagMAX™
Express-96 Magnetic Particle Processor helps to increase throughput and
efficiency. Using the automated system, host cell residual DNA can be
isolated from up to 24 triplicate test samples, typically in less than 2 hours.
Prepare Samples Products
- Quick Reference Card: resDNASEQ™ Quantitative CHO DNA Kit
- Protocol: PrepSEQ™ Residual DNA Sample Preparation Kit
- Protocol: resDNASEQ™ Quantitative CHO DNA
- Quick Reference Card: PrepSEQ™ Residual DNA Sample Preparation Kit
- Product Bulletin: CHO Residual DNA Quantitation
- Solutions for the Biopharmaceutical Industry
Have questions about Gibco® media, POROS® chromatography resins, Applied Biosystems® SEQ rapid contaminant tests, or our Cell Therapy products? Click below to access product brochures, training videos, mobile apps, and more.
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