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Cloning

Ultimate™ ORF Clones

Fast Track to Expression and Analysis
  • Vast selection – over 16,000 Ultimate™ Human ORF Clones and over 2,500 Ultimate™ Mouse ORF Clones
  • Gateway® entry clone format – 1-hour cloning into expression vectors gets you to expression and analysis faster
  • 100% amino acid match guarantee – sequence verification against GenBank®, Ensembl, and Swissprot databases
  • Quality Tested & Guaranteed
    Each clone in the Ultimate™ ORF Clone Collection is guaranteed to match GenBank® sequence information 100% at the amino acid level. To ensure that you receive the highest-quality and most scientifically relevant clones, Ultimate™ ORF clones have been full-insert sequenced. In addition, before shipping, the clone culture must pass a stringent QC test which authenticates the identity of the clone. You can have peace of mind knowing the clone you ordered is the clone you will receive.

    Ready to Go
    Ultimate™ ORF clones eliminate the initial cloning and verification steps of your gene discovery research. There is no need to perform tedious RNA isolation, cDNA synthesis, PCR amplification, cloning, sequencing, and validation procedures. You will save literally weeks of time by moving directly to the expression and protein analysis steps of your workflow.

    All Ultimate™ ORFs are provided in the Gateway® entry vector pENTR™221. This means you can rapidly and efficiently recombine the ORF of interest into any expression vector and go straight to gene analysis in your system of choice. Simply choose from Invitrogen's comprehensive selection of Gateway®-compatible expression vectors (destination vectors) or convert your vector of interest into a destination vector using the Gateway® Vector Conversion Kit.

    Ordering
    All Ultimate™ ORF clones can be readily identified and ordered via an online search using the Ultimate™ ORF Browser. This convenient search engine allows you to find your gene of interest using keywords, clone ID, accession number, or sequence information.

    Want to also locate in-depth, updated gene and protein information on your specific clone of interest? Simply click on the clone ID hyperlink to retrieve the Ultimate™ ORF Card for that specific clone. Ultimate™ ORF Cards are dynamically linked to public databases, bringing together all the information you need on your ORF clone. Ultimate™ ORF Cards contain continuously updated information on:
    • Splice variants
    • SNPs
    • Gene symbol
    • Gene ontology
    • Amino acid sequence
    • Protein domains
    • Sequence alignments
    • Molecular weight
    • Protease digest patterns
    • Structure detemination

    What You Receive
    Your clone will be shipped as a glycerol stock on dry ice.  Please store promptly at -80°C.

    Find your gene of interest today! - Visit our Ultimate ORF Browser

Disclaimer

  • Invitrogen™ Corporation provides an evolving clone collection where each gene represented is identified as containing a complete open reading frame, based on a dynamic source of bioinformatic information contained within GenBank. GenBank is a genetic sequence database, which contains an annotated collection of all publicly available DNA sequences.

    Ultimate™ ORF Clones are typically derived from cDNA clones believed to contain a complete open reading frame, according to the most current GenBank update. Please note, subsequent GenBank updates may show some of these cDNA clones to contain a partial sequence, a non-coding sequence or other artifacts.

    Quarterly, Invitrogen™ Corporation will update the Ultimate™ ORF Clone Collection, by adding, deleting, or modifying the bioinformatic information to more accurately reflect the most current scientific information associated with each of the clones represented in the collection.

    It is highly recommended that the purchaser carefully evaluate the ORF clone sequence prior to purchase. It is the purchaser’s responsibility to check whether the sequence associated with any particular clone meets his/her scientific requirement prior to purchase.

    Invitrogen makes no warranties, express or implied, that the manufacture, use, importation, or sale of the gene contained within any Ultimate™ ORF clone will not infringe any third party intellectual property right.