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Association of calnexin with mutant peripheral myelin protein-22 ex vivo: a basis for
Citations & References
- Authors: Dickson K M; Bergeron J J M; Shames I; Colby J; Nguyen D T; Chevet E; Thomas D Y; Snipes G J;
- Journal: Proc Natl Acad Sci U S A (2002) 99:9852-9859
- Product Usage: Sciatic nerve and liver tissues from either wt, Tr-J/+, or Tr-J/Tr-J mice were pooled and homogenized in Trizol (Invitrogen). Total RNA was isolated and converted into cDNA by using oligo(dT)20-primed oligonucleotides and the Thermoscript RT-PCR system (Invitrogen).
- ID: PN66098
743a3892f8ced95e1fab16fc98a9f3e3_Citations
Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase.
Citations & References
- Authors: Fehlner-Gardiner Christine; Roshick Christine; Carlson John H; Hughes Scott; Belland Robert J; Caldwell Harlan D; McClarty Grant;
- Journal: J Biol Chem (2002) 277:26893-27796
- Product Usage: HeLa 229 cells were infected with C. trachomatis. As a negative control, a mock-infected flask was prepared in the same manner but without the addition of EBs. The cells were incubated for 24 h at 37 °C, and then total RNA was prepared using Trizol. After treatment with amplification-grade DNase I, 1 µg of RNA was reverse-transcribed using random hexamer primers and Thermoscript reverse transcriptase and then treated with RNase H.
- ID: PN62445
d0881bf5a7895640b88372a784a7eec4_Citations
A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells.
Citations & References
- Authors: Holt Jeffrey R; Gillespie Susan K H; Provance D William; Shah Kavita; Shokat Kevan M; Corey David P; Mercer John A; Gillespie Peter G;
- Journal: Cell (2002) 108:371-452
- Product Usage: We purified total RNA from utricles of genotyped mice, synthesized cDNA using oligo(dT)-containing primers, and carried out PCR (ThermoScript RT-PCR, Gibco-BRL).
- ID: PN60971
- Catalog #s
- 11146024
3333c340e3fbea852ff31221049db563_Citations
FCP1 phosphorylation by casein kinase 2 enhances binding to TFIIF and RNA polymerase II carboxyl-terminal domain phosphatase activity.
Citations & References
- Authors: Palancade B, Dubois MF, Bensaude O,
- Journal: J Biol Chem (2002) 277:36061-36067
- Product Usage: RNA isolated from A6 cells was used to amplify the xRAP74 open reading frame using Thermoscript reverse transcriptase.
- ID: PN62366
- Catalog #s
- 12236014
9c9050ce6b23581dbe705de1479791c0_Citations
Expression of Th1-mediated immunity in mouse lungs induces a Mycobacterium tuberculosis transcription pattern characteristic of nonreplicating persistence.
Citations & References
- Authors: Shi L, Jung YJ, Tyagi S, Gennaro ML, North RJ,
- Journal: Proc Natl Acad Sci U S A (2003) 100:241-246
- Product Usage: Reverse transcription was performed with gene-specific primers and ThermoScript transcriptase (Invitrogen) according to the manufacturer's recommendations
- ID: PN62982
- Catalog #s
- 11146016
82ce994baf8f5e47f62f12b35adecf03_Citations
Shielding of the A1 Domain by the D′D3 Domains of von Willebrand Factor Modulates Its Interaction with Platelet Glycoprotein Ib-IX-V.
Citations & References
- Authors: Ulrichts H, Udvardy M, Lenting PJ, Pareyn I, Vandeputte N, Vanhoorelbeke K, Deckmyn H,
- Journal: J Biol Chem (2006) 281:4699-4707
- Product Usage: The authors used the ThermoScript RT-PCR system and oligo dT (20) to isolate the cDNA for the 1C1E7 anti-VWF monoclonal antibody. The pCR-Blunt TOPO and pCR2.1 TOPO vectors were used to clone PCR products in different stages of the project. The 1C1E7scFv gene was cloned in pBAD TOPO TA vector for expression of the fusion protein in E.coli and in pSegTag/FRT/V5His for mammalian expression.
- ID: PN64111
62161512d8b1b5db826778917e974b21_Citations
Analysis of gene expression profiles in normal and neoplastic ovarian tissue samples identifies candidate molecular markers of epithelial ovarian cancer.
Citations & References
- Authors: Welsh JB, Zarrinkar PP, Sapinoso LM, Kern SG, Behling CA, Monk BJ, Lockhart DJ, Burger RA, Hampton GM
- Journal: Proc Natl Acad Sci U S A (2001) 98:1176-1181
- Product Usage: Reverse Transcription (RT)-PCR. One microgram of total RNA from selected normal and tumor tissue samples was reverse transcribed with random hexamers and thermostable TaqGold polymerase by using the Thermoscript RT-PCR System. One microliter of RT product was amplified by primer pairs specific for HE4, CD24, and LU(BCAM).
- ID: PN60457
547a5a56d43f6d40f9a6a2f76501d013_Citations
Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia.
Citations & References
- Authors: Rawat VP, Cusan M, Deshpande A, Hiddemann W, Quintanilla-Martinez L, Humphries RK, Bohlander SK, Feuring-Buske M, Buske C,
- Journal: Proc Natl Acad Sci U S A (2004) 101:817-822
- Product Usage: First-strand cDNA was synthesized from 1 µg of total RNA by using the ThermoScript RT-PCR system. A PCR product was cloned in frame to the 3' end of the histidine epitope of pCDNA6/ His A plasmid.
- ID: PN62537
13bf4a96378f3854bcd9792d132eff9f_Citations
SIZ1 small ubiquitin-like modifier E3 ligase facilitates basal thermotolerance in Arabidopsis independent of salicylic acid.
Citations & References
- Authors: Yoo CY, Miura K, Jin JB, Lee J, Park HC, Salt DE, Yun DJ, Bressan RA, Hasegawa PM,
- Journal: Plant Physiol (2006) 142:1548-1558
- Product Usage: PureLink Micro-to-midi Total RNA Purification System was used to isolate and purify total RNA from 10-day old seedlings of Arabidopsis (small flowering plants related to cabbage and mustard). The RNA was then used template for making cDNAs using Thermoscript RT-PCR system.
- ID: PN66308
7eab47bf3a57db8e440e5a788467c37f_Citations
Regulation of gonadotropin gene expression by Mullerian inhibiting substance.
Citations & References
- Authors: Bedecarrats GY, O'Neill FH, Norwitz ER, Kaiser UB, Teixeira J,
- Journal: Proc Natl Acad Sci U S A (2003) 100:9348-9353
- Product Usage: LbT2 and aT3-1 cells were cultured in high-glucose DMEM containing 10% female FCS and 1% penicillin/streptomycin mix. For Northern blot analysis, total RNA was isolated by using TRIzol. 250 ng of Total RNA was used with FAM labelled LUX primers with Platinum Quantitative RT-PCR Thermoscript kit on a SmartCycler(Cepheid, Sunnyvale, CA.
- ID: PN65319
40db0c1f0e46163f486e6d089a2d0684_Citations
ATP stimulates signal recognition particle (SRP)/FtsY-supported protein integration in chloroplasts.
Citations & References
- Authors: Yuan Jianguo; Kight Alicia; Goforth Robyn L; Moore Misty; Peterson Eric C; Sakon Joshua; Henry Ralph;
- Journal: J Biol Chem (2002) 277:32400-32404
- Product Usage: The coding sequence for the precursor form of cpFtsY was amplified from Arabidopsis RNA by reverse transcription-PCR using ThermoScript reverse transcriptase and ligated into pGEM-4Z vector. Expression plasmids for HisLHCP, HiscpSRP43, and Trx-cpFtsY were transformed into Escherichia coli strain BL21*, and cells were cultured in LB medium. Cells were induced with 0.5 mM or with IPTG plus 0.2% L-(+)-arabinose.
- ID: PN65369
62509b585210b8dd3e871ca3c23f186b_Citations
Soluble and transmembrane isoforms of novel interleukin-17 receptor-like protein by RNA splicing and expression in prostate cancer.
Citations & References
- Authors: Haudenschild Dominik; Moseley Timothy; Rose Larry; Reddi A Hari;
- Journal: J Biol Chem (2002) 277:4309-4325
- Product Usage: cDNA was made from 2 µg of random primed total RNA using ThermoScript Reverse Transcription System.
- ID: PN60220
- Catalog #s
- 11146024
baaf111c2bdc129a356a14f79fefd224_Citations
Divergent regulation of the growth-promoting gene IEX-1 by the p53 tumor suppressor and Sp1.
Citations & References
- Authors: Im Hee-Jeong; Pittelkow Mark R; Kumar Rajiv;
- Journal: J Biol Chem (2002) 277:14612-14633
- Product Usage: Reverse transcription-PCR for the construction of Sox18 cDNA was performed using the ThermoScript reverse transcription-PCR system .
- ID: PN61182
- Catalog #s
- 12236014
b79be635901b01b11a2eadb9a587532b_Citations
Role of Lkb1, the causative gene of Peutz-Jegher′s syndrome, in embryogenesis and polyposis.
Citations & References
- Authors: Jishage Kou-ichi; Nezu Jun-ichi; Kawase Yosuke; Iwata Takamitsu; Watanabe Miho; Miyoshi Akio; Ose Asuka; Habu Kiyoshi; Kake Takei; Kamada Nobuo; Ueda Otoya; Kinoshita Michiko; Jenne Dieter E; Shimane Miyuki; Suzuki Hiroshi;
- Journal: Proc Natl Acad Sci U S A (2002) 99:8903-8911
- Product Usage: cDNA was synthesized from total RNA of the glandular stomach of wild-type and Lkb1+/ mice by using the Thermoscript reverse transcriptase-PCR system.
- ID: PN60652
- Catalog #s
- 12236022
1c21b0912f1088c26479be9299203f2b_Citations
A novel 43-kDa protein as a negative regulatory component of phenoloxidase-induced melanin synthesis.
Citations & References
- Authors: Zhao M, Söderhäll I, Park JW, Ma YG, Osaki T, Ha NC, Wu CF, Söderhäll K, Lee BL,
- Journal: J Biol Chem (2005) 280:24744-24751
- Product Usage: Melanization-inhibiting protein (MIP) was expressed using Bac-to-Bac Baculovirus expression sytem. The cDNA encoding the mature MIP was subcloned into pFastBacDUAL vector. The recombinant virus was amplified using Sf9 cells in SF-900II serum-free medium. To produce the protein, High Five cells were grown in Grace's medium supplemented with 20% Sf-900II serum-free medium and 0.1% Pluonic F-68 reagent. TRIzol and ThermoScript Reverse Transcriptase were for RT-PCR.
- ID: PN65016
28a7602724ba16600d5ccc644c19bf18_Citations
