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Reverse Transcriptase Enzymes
Invitrogen SuperScript® III Reverse Transcriptase is the most widely used brand of enzyme for cDNA synthesis, due to the proven ability to deliver reliable and consistently high yields of fully representative full length cDNA.
SuperScript® II Reverse Transcriptase View 66 Citations
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| 18064014 | 10,000 units | 281.00 |
Description
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single- View full product details >
General Specifications
- Optimal Reaction Temperature
-
42° C
- Final Product Size(s)
-
12.3 kb or less
- Ribonuclease H Activity
-
Reduced
- Concentration
-
200 U⁄µl
- Reverse Transcriptase
-
SuperScript® II
- Form
-
Frozen
- Format
-
Tube(s)
- Template
-
ssRNA
RNA-DNA Hybrid
ssDNA
- Sensitivity
-
Medium
- Product Size
-
10,000 units
- Enzyme Function
-
RNA dependent DNA Polymerase
- Shipping Condition
-
Wet Ice
- Number of Reactions
-
50 Reactions
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- PCR & Real-Time PCR
User Ratings
Rate this productSuperScript® II Reverse Transcriptase View 150 Citations
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| 18064022 | 2,000 units | 83.00 |
Description
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single- View full product details >
General Specifications
- Optimal Reaction Temperature
-
42° C
- Final Product Size(s)
-
12.3 kb or less
- Ribonuclease H Activity
-
Reduced
- Concentration
-
200 U⁄µl
- Reverse Transcriptase
-
SuperScript® II
- Form
-
Frozen
- Format
-
Tube(s)
- Quantity
-
2000 units
- Template
-
RNA-DNA Hybrid
ssRNA
ssDNA
- Sensitivity
-
Medium
- Product Size
-
2,000 units
- Enzyme Function
-
RNA dependent DNA Polymerase
- Shipping Condition
-
Wet Ice
- Number of Reactions
-
10 Reactions
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- PCR & Real-Time PCR
User Ratings
SuperScript® II Reverse Transcriptase View 30 Citations
| Catalog # | Unit Size | List Price | |
|---|---|---|---|
| 18064071 | 4 × 10,000 units | 1000.00 |
Description
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single- View full product details >
General Specifications
- Optimal Reaction Temperature
-
42° C
- Final Product Size(s)
-
12.3 kb or less
- Ribonuclease H Activity
-
Reduced
- Concentration
-
200 U⁄µl
- Reverse Transcriptase
-
SuperScript® II
- Form
-
Frozen
- Format
-
Tube(s)
- Template
-
ssRNA
ssDNA
RNA-DNA Hybrid
- Sensitivity
-
Medium
- Product Size
-
4 × 10,000 units
- Enzyme Function
-
RNA dependent DNA Polymerase
- Number of Reactions
-
200 Reactions
- Regulatory Statement
-
For Research Use Only. Not for use in diagnostic procedures.
Product Applications
- PCR & Real-Time PCR
User Ratings
SuperScript® Reverse Transcriptase
Overview
Choose the right Reverse Transcriptase: SuperScript® II or SuperScript® III, maximum sensitivity for real time RT-PCR and microarray experiments.
SuperScript® III Reverse Transcriptase
Overview
SuperScript® III Reverse Transcriptase (RT) provides higher yields of full-length cDNA for more complete gene product representation compared to other RT enzymes. Robust cDNA
Is it necessary to digest first-strand cDNA made with SuperScript® III or II Reverse Transcriptase (RT) or ThermoScript™ RT with RNase H?
Product FAQ
It is not always necessary to digest the first-strand cDNA with RNase H. For many primer-template combinations, PCR products are seen without the RNase H treatment. Since SuperScript® II and III RT lack RNase H and ThermoScript™ RT essentially is RNase H minus, the un-nicked RNA/cDNA hybrids may not denature well during the initial denaturation steps in PCR, leading to decreased sensitivity of the PCR reaction. These cDNA templates may require RNase H digestion. If a PCR product is not obtained when an RNase H step is not included after cDNA synthesis, always repeat the PCR after an RNase H treatment.
Answer Id: 2915
SuperScript II Reverse Transcriptase
Manual / Product Insert
What are the differences between AMV Reverse Transcriptase (RT), MMLV RT, SuperScript® RT, SuperScript® II and III RT, Thermo-X™ RT, and ThermoScript™ RT?
Product FAQ
AMV RT is an RT enzyme from avian myeloblastosis virus that can use single-stranded RNA or DNA as a template in the presence of a primer to synthesize the complementary DNA strand. The optimal reaction temperature is 42 degrees C. AMV RT has an RNase H activity and is inhibited by ribosomal and transfer RNA.
M-MLV RT is an RT enzyme from Moloney Murine Leukemia Virus that can use single-stranded RNA or DNA as a template in the presence of a primer to synthesize the complementary DNA strand. This enzyme has RNase H activity, although at a lower level than AMV-RT. M-MLV RT does not have an endonuclease activity. The optimal reaction temperature for this enzyme is 37 degrees C and it is less sensitive than AMV RT to inhibition by rRNA and tRNA. It can generate cDNA up to 9.5 kb.
SuperScript® RT is a modified M-MLV RT. It completely lacks the RNase H activity due to a deletion from the holoenzyme. SuperScript® RT is active up to 50 degrees C with an optimal reaction temperature of 45 degrees C.
SuperScript® II RT also lacks the RNAse H activity of M-MLV RT due to point mutations in the RNAse H domain. SuperScript® II RT yields more full length cDNA than SuperScript® RT since it retains the same level of polymerase activity as the parent M-MLV RT. SuperScript® II RT can be used up to 50 degrees C for cDNA synthesis. The optimal temperature for the enzyme is 37-45 degrees C.
SuperScript® III RNase H- RT is a proprietary point mutant of SuperScript® II RNase H- RT that has increased thermostability and longer half-life than any other RT for overall better performance. It is active at 50 degrees C and has a half-life of 220 min at this temeperature, providing the highest cDNA yield and increased specificity with GSP of all RTs, making it ideal for RT-PCR for a specific gene or generating cDNA from total RNA sample. The enzyme is used to synthesize first-strand cDNA at temperatures up to 55 degrees C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 kb.
ThermoScript™ RT is a recombinant avian reverse transcriptase that is lacking essentially all RNase H activity. It has been engineered to have high thermal stability (up to 70 degrees C) and is a good choice for RT-PCR of exceptionally difficult templates. It displays excellent specificity, yield and sensitivity in RT PCR. It can generate cDNA from 100 bp to 12 kb.
Thermo-X™ RT is a highly thermostable enzyme that provides full-length, first-strand cDNA synthesis at temperatures up to 70 degrees C. The enzyme is purified from E. coli containing genes from a proprietary thermophilic eubacteria. Thermo-X™ RT has a half-life of 120 minutes at 65 degrees C, and can synthesize cDNA from targets up to 12 kb in length.
Answer Id: 2956
SuperScript® II Reverse Transcriptase
MSDS
SuperScript® II Reverse Transcriptase
MSDS
SuperScript® II Reverse Transcriptase
MSDS
Reverse Transcriptase Enzymes
Overview
Invitrogens SuperScript® III Reverse Transcriptase is the most widely used brand of enzyme for cDNA synthesis, due to the proven ability to deliver reliable and consistently high
SuperScriptâ„¢ Indirect cDNA Labeling System | Life Technologies
Experiment Protocol
SuperScript® One-Cycle cDNA Kit
Overview
Maintain consistency without downtime in your Affymetrix GeneChip® workflow SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle AssaysOrder Now
